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Gesa Langer, Wolfgang Jagla, Wolfgang Behrens–Baumann, Sven Walter, Werner Hoffmann; Secretory Peptides TFF1 and TFF3 Synthesized in Human Conjunctival Goblet Cells. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2220-2224.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. The objective of this study was to determine whether human conjunctival
epithelium synthesizes TFF-peptides (formerly P-domain peptides,
trefoil factors), a family of mucin-associated secretory peptides of
the gastrointestinal tract.
methods. Expression of TFF-peptides in human conjunctiva was monitored by
reverse transcription–polymerase chain reaction analysis. Antisera
specific for TFF-peptides were used for immunohistochemistry to
determine the presence and distribution of TFF-peptides in human
results. mRNA expression of TFF1 and TFF3, but not TFF2, was detected in human
conjunctiva. TFF1 and TFF3 but not TFF2 are stored in conjunctival
goblet cells only as revealed by immunofluorescence.
conclusions. Goblet cells of the human conjunctiva synthesize TFF1 and TFF3. These
peptides, together with the secretory ocular mucin MUC5AC, may
contribute to the rheological properties of the tear film. They also
may influence healing of corneal wounds due to their motogenic
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