Purchase this article with an account.
Xiaolin Gu, Sara Samuel, Mohamed El-shabrawey, Ruth B. Caldwell, Manuela Bartoli, Dennis M. Marcus, Steven E. Brooks; Effects of Sustained Hyperoxia on Revascularization in Experimental Retinopathy of Prematurity. Invest. Ophthalmol. Vis. Sci. 2002;43(2):496-502.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To investigate the effects of prolonged hyperoxia on vascular recovery
and glia survival after experimentally induced retinopathy of
prematurity (ROP) in the mouse.
methods. The effects of hyperoxia on revascularization and vitreous
neovascularization were compared between mice raised in 75% oxygen
from postnatal day (P)7 to P12, followed by room air recovery and mice
raised in 75% oxygen from P7 to P27. The status of astrocytes and
Müller cells was evaluated by glial fibrillary acidic protein
(GFAP) immunohistochemistry on retinal wholemounts and serial sections.
A window of susceptibility to oxygen-induced vaso-obliteration was
defined by comparing the extent of retinal vaso-obliteration resulting
from 2 days of hyperoxia beginning on P7, P9, P11, P13, or P15.
results. Oxygen-induced vaso-obliteration of retinal capillaries was limited to
the period between birth and P15. Paradoxically, revascularization was
markedly accelerated and neovascularization markedly reduced in mice
maintained in prolonged hyperoxia (P7–P27) compared with mice
recovering in room air. The extended use of 75% oxygen during the
recovery period was associated with preservation of astrocytes and
Müller cells in the avascular retina.
conclusions. The antiangiogenic effect of hyperoxia on retinal capillaries is
strongly dependent on postnatal age. A protocol of continuous 75%
supplemental oxygen accelerates recovery of inner retinal vasculature
and prevents vitreous neovascularization, by a mechanism that may
involve preservation of inner retinal glia.
This PDF is available to Subscribers Only