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Katsuyoshi Suzuki, Toshiro Tanaka, Miho Enoki, Teruo Nishida; Coordinated Reassembly of the Basement Membrane and Junctional Proteins during Corneal Epithelial Wound Healing. Invest. Ophthalmol. Vis. Sci. 2000;41(9):2495-2500.
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purpose. To characterize changes in the localizations of the basement membrane
protein laminin-1 and of adhesion proteins of intercellular junctions
during wound healing after epithelial ablation in the rat cornea.
methods. Epithelial ablation was performed with an excimer laser. Rats were
killed immediately, 12 hours, 24 hours, 3 days, or 4 weeks after
ablation, and corneal cryosections were subjected to two-color
immunofluorescence staining with antibodies to laminin-1 and antibodies
to connexin43 for gap junctions, desmoglein 1 or 2 (desmoglein 1 + 2)
for desmosomes, or E-cadherin for adherens junctions. Sections were
also stained with antibodies to occludin for examination of tight
results. Laminin-1 was detected in the basement membrane, connexin43 in the
basal cell layer, desmoglein 1 + 2 in the wing cell layer, E-cadherin
in all cell layers, and occludin in the wing and superficial cell
layers of the intact corneal epithelium. Laminin-1 immunostaining was
not detected at the leading edge of migrating epithelial cells until 24
hours after ablation. Expression of connexin43 and desmoglein 1 + 2
coincided with the reappearance of laminin-1, whereas that of
E-cadherin and occludin was apparent regardless of the absence or
presence of laminin-1. Epithelial remodeling was complete after 4
weeks. The basement membrane was re-established, and the expression
patterns for all the adhesion proteins were identical with those
characteristic of the intact cornea.
conclusions. Actively migrating epithelial cells no longer manifested gap junctions
and desmosomes in the wounded area with no basement membrane.
Re-establishment of the basement membrane coincided with reassembly of
these intercellular junctions, suggesting that the presence of the
basement membrane may be required for their reformation in the rat
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