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Akihiro Nishida, Masayo Takahashi, Hidenobu Tanihara, Ichiro Nakano, Jun B. Takahashi, Akira Mizoguchi, Chizuka Ide, Yoshihito Honda; Incorporation and Differentiation of Hippocampus-Derived Neural Stem Cells Transplanted in Injured Adult Rat Retina. Invest. Ophthalmol. Vis. Sci. 2000;41(13):4268-4274.
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purpose. In a previous study it has been shown that adult rat
hippocampus-derived neural stem cells can be successfully transplanted
into neonatal retinas, where they differentiate into neurons and glia,
but they cannot be transplanted into adult retinas. In the current
study, the effect of mechanical injury to the adult retina on the
survival and differentiation of the grafted hippocampal stem cells was
methods. Mechanical injury was induced in the adult rat retina by a hooked
needle. A cell suspension (containing 90,000 neural stem cells) was
slowly injected into the vitreous space. The specimens were processed
for immunohistochemical studies at 1, 2, and 4 weeks after the
results. In the best case, incorporation of grafted stem cells was seen in 50%
of the injured retinas. Most of these cells located from the
ganglion cell layer through the inner nuclear layer close to the injury
site. Immunohistochemically, at 1 week, more than half of the grafted
cells expressed nestin. At 4 weeks, some grafted cells showed
immunoreactivity for microtubule-associated protein (MAP) 2ab, MAP5,
and glial fibrillary acidic protein (GFAP), suggesting progress in
differentiation into cells of neuronal and astroglial lineages.
However, they showed no immunoreactivity for HPC-1,
calbindin, and rhodopsin, which suggests that they did not
differentiate into mature retinal neurons. Immunoelectron microscopy
revealed the formation of synapse-like structures between graft and
conclusions. By the manipulation of mechanical injury, the incorporation and
subsequent differentiation of the grafted stem cells into neuronal and
glial lineage, including the formation of synapse-like structures, can
be achieved, even in the adult rat retina.
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