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Ajit B. Majji, Jingtai Cao, Kwang Yul Chang, Atsushi Hayashi, Seema Aggarwal, Rhonda R. Grebe, Eugene de Juan; Age-Related Retinal Pigment Epithelium and Bruch’s Membrane Degeneration in Senescence-Accelerated Mouse. Invest. Ophthalmol. Vis. Sci. 2000;41(12):3936-3942.
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purpose. To investigate age-related changes in the retinal pigment epithelium
(RPE), Bruch’s membrane, and choriocapillaris in the
senescence-accelerated mouse (SAM).
methods. The external and eyecup features and light and electron microscopic
findings were examined in three male and two female mice of a
senescence-prone mouse strain (SAM P8) monthly for 12
months. These results were compared with those in age-matched mice of
similar background but senescence resistant (SAM R1).
Choroidal vascular casts were prepared at 12 months in seven mice each
of the SAM P8 and SAM R1 strains. Quantitative
analysis of area of choriocapillaris was performed by automated image
analysis, and the results were analyzed by paired Student’s t-test.
results. We found in the SAM P8 strain that hair loss, coarseness of
hair texture, and ulceration of skin appeared and increased as the age
advanced (at approximately 5–9 months). Eyecup examination showed no
differences. Light and electron microscopy revealed progressively more
prominent abnormalities in the RPE and Bruch’s membrane mice older
than 10 months. Two of the five SAM P8 mice older than 11
months showed what appeared to be intra-Bruch’s membrane choroidal
neovascularization. The RPE and Bruch’s membrane appeared normal in
the SAM R1 strain. In the SAM P8, vascular
casts of the choriocapillaris showed a mild but significant decrease in
vascular area when compared with the SAM R1 strain at 12
months (P = 0.011).
conclusions. Senescence accelerated mice develop progressive age-related changes in
the RPE–Bruch’s–choriocapillaris complex that have features that may
be relevant in the study of age-related macular changes in
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