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Dao-Yi Yu, Stephen J. Cringle, Er-Ning Su, Paula K. Yu; Intraretinal Oxygen Levels before and after Photoreceptor Loss in the RCS Rat. Invest. Ophthalmol. Vis. Sci. 2000;41(12):3999-4006.
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purpose. To measure the intraretinal oxygen environment at different stages in
the Royal College of Surgeons (RCS) rat model of retinal degeneration
to determine whether changes in oxygen level are an important aspect of
methods. Oxygen-sensitive microelectrodes were used to measure oxygen tension as
a function of depth through the retina of anesthetized, mechanically
ventilated RCS rats at ages ranging from postnatal day (P)20 to P104.
The oxygen profiles were correlated with histologic observations of the
cellular changes within the dystrophic retinas and compared with those
in RCS-rdy + control animals and published values
in normal mature rats.
results. Although the youngest rats studied exhibited some differences in
intraretinal oxygen distribution compared with mature animals, the
distribution in dystrophic RCS rats at P20 was not significantly
different from that in age-matched control subjects. However, the
intraretinal oxygen distribution in dystrophic RCS rats was clearly
affected after approximately P30, reflecting a loss of photoreceptor
oxygen consumption consistent with histologic observations. In
contrast, oxygen uptake by the inner retina was still evident long
after the loss of photoreceptors was essentially complete.
conclusions. There was no significant tissue hypoxia during photoreceptor
degeneration in the dystrophic RCS rat. The changes in intraretinal
oxygen distribution are consistent with the loss of outer retinal
oxygen uptake but the preservation of inner retinal oxygen
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