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Adriana Di Polo, Li Cheng, Garth M. Bray, Albert J. Aguayo; Colocalization of TrkB and Brain-Derived Neurotrophic Factor Proteins in Green-Red–Sensitive Cone Outer Segments. Invest. Ophthalmol. Vis. Sci. 2000;41(12):4014-4021.
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purpose. To examine the distribution of neurotrophins (NTs) and their catalytic
receptors in adult rat photoreceptors.
methods. Immunocytochemistry and Western blot analyses were performed using
primary antibodies raised against NTs (nerve growth factor [NGF],
brain-derived neurotrophic factor [BDNF], NT-3, and NT-4/5) and NT
receptors (TrkA, TrkB, TrkC, and p75NTR). Double-labeling of retinal
sections with opsin-specific antibodies was performed to identify each
photoreceptor type. Competitive experiments using excess recombinant NT
or Trk receptors confirmed the binding specificity of each antibody.
results. TrkB and BDNF immunoreactivity was colocalized in cone outer segments.
TrkB and BDNF were detected in all green-red–sensitive cones, but not
in blue-UV cones or rods, and other NTs and NT receptors were not
detected in any of the photoreceptor types.
conclusions. The findings suggest a specific role for BDNF through its signaling
receptor TrkB in the function and maintenance of green-red cones, the
predominant cone type in the rat retina.
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