Purchase this article with an account.
Sung Zoo Kim, Hun Sook Kim, Kyung Sun Lee, Sook Jeong Lee, Kyung Hwan Seul, Gou Young Koh, Kyung Woo Cho, Suhn Hee Kim; Coexistence of C-Type Natriuretic Peptide and Atrial Natriuretic Peptide Systems in the Bovine Cornea. Invest. Ophthalmol. Vis. Sci. 2000;41(9):2671-2677.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To determine whether the cornea synthesizes natriuretic peptides and
contains their receptors.
methods. The synthesis of the natriuretic peptides, C-type natriuretic peptide
(CNP) and atrial natriuretic peptide (ANP), in the bovine cornea was
determined by high-performance liquid chromatography (HPLC) with
radioimmunoassay and Southern blot analysis. The presence of
natriuretic peptide receptor (NPR)-A and -B and their localizations
were measured by reverse transcription–polymerase chain reaction
(RT-PCR), in vitro autoradiography, and the activation of particulate
guanylyl cyclase by natriuretic peptides in the corneal membrane.
results. The serial dilution curves of corneal extracts were parallel to the
standard curves of CNP and ANP. With reversed-phase HPLC, a major
immunoreactive peak of CNP or ANP was observed at the elution time
corresponding with synthetic CNP(1-53) or atriopeptin III
(APIII), respectively. The presence of mRNAs of CNP and ANP was also
detected in the cornea by RT-PCR and/or Southern blot analysis.
Production of 3′,5′-cyclic guanosine monophosphate (cGMP) by the
activation of particulate guanylyl cyclase in the corneal membrane was
stimulated by ANP, BNP, and CNP. More cGMP was produced by CNP than by
the other natriuretic peptides. Specific 125I-[Tyr0]-CNP(1-22) binding
sites were localized in the endothelial cell layer of cornea. The
apparent dissociation constant (K d) value of
the cornea was 3.06 ± 0.73 nM and the maximum binding capacity
was 3.40 ± 0.63 femtomoles/mm2. Both NPR-A and NPR-B
mRNAs were detected by RT-PCR.
conclusions. The cornea synthesizes CNP and ANP and contains their receptors. These
results suggest that the CNP and ANP systems coexist in the bovine
This PDF is available to Subscribers Only