Abstract
purpose. The Ocular Response Analyzer (ORA) is a new instrument that measures the corneal biomechanical response (corneal hysteresis, CH) to rapid indentation by an air jet. CH is the difference in applanation pressures (P1, P2) between the rising and falling phases of the air jet. The investigation had two parts: a characterization study and a validation study. In the characterization study, the purposes were to investigate the intraocular pressure (IOP)–dependence of CH and to characterize the performance of the ORA. In the validation study, the purposes were to investigate the association between CH and both age and central corneal thickness (CCT) and the agreement between ORA and Goldmann applanation tonometer (GAT) IOP measurements.
methods. For the characterization study, data were collected from 105 untreated subjects (45 ocular hypertensive patients and 60 normal subjects; mean age, 60 years, range, 26–82). GAT and ORA measurements were performed before and after IOP lowering of one randomly selected eye with apraclonidine drops. The change in P1 and P2 (arbitrary units) in relation to change in GAT IOP was analyzed to calibrate the instrument. The relation between P1, P2, and CCT was explored and ORA IOP was derived from the analyses. For the validation study, ORA and GAT IOP and CCT were measured in 144 eyes of 144 untreated subjects (mean age, 58 years; range, 19–83). The characterization calculations were applied to the dataset and values of CH and ORA IOP were calculated. The relationship between CH and both subject age and CCT was determined. The associations between CH and CCT and between ORA and GAT IOPs, were investigated by linear regression analysis. The agreement between measuring devices was calculated.
results. In the characterization study, P1 changed by 6.41 arbitrary units for every 1-mm Hg change in GAT IOP. CH (P1 − P2) changed by −1.60 arbitrary units for every 1-mm Hg change in GAT IOP. For each unit change in P2, P1 changed by 1.27 units. From this association a new IOP-independent corneal factor was derived [P1 − (P2/1.27)] and is termed the corneal constant factor (CCF; mm Hg). ORA IOP normalized for CCF was defined as P2 – CCF (mm Hg). The CCF (mm Hg) was associated with CCT (micrometers) and with age: CCF = [(0.036 · CCT) − (0.028 · age)] + 1.06 (adjusted r 2 = 0.34; P < 0.0001 for CCT, P = 0.007 for age). Normalized ORA IOP measurements were not associated with CCT. GAT IOP was associated with CCT and CCF—more strongly with the latter: GAT IOP = (0.03 · CCT)+1.52 (r 2 = 0.06, P = 0.002); GAT IOP = (0.65 · CCF) + 4.5 (r 2 = 0.13, P < 0.0001). The mean difference (95% limits of agreement) between GAT and normalized ORA IOP was 0.1 (−6.6 to +6.8) mm Hg.
conclusions. The CCF describes an IOP-independent biomechanical property of the cornea that increases with thicker CCT and decreases with greater age. It is moderately strongly associated with CCT and yet explains more of the interindividual variation in GAT IOP than does CCT. Normalized ORA IOP measurements are not associated with CCT.
Glaucoma can be defined as a progressive optic neuropathy, with characteristic morphologic changes of the optic nerve head and retinal nerve fiber layer in the absence of other ocular disease and congenital abnormalities.
1 Raised intraocular pressure (IOP) is the major modifiable risk factor for the development
2 and progression
3 of the disease. The Goldmann applanation tonometer (GAT) is currently the gold standard for measuring IOP.
When Goldmann and Schmidt introduced the GAT in 1957, they acknowledged that a variation in central corneal thickness (CCT) would influence IOP readings, but stated that in the absence of corneal disease, the CCT did not vary much around 500 μm. They also assumed that the effects of corneal rigidity would be counterbalanced by the surface tension of the tear meniscus’s drawing the tonometer tip onto the cornea when applanating a circular area of diameter 3.06 mm.
4 However, Von Bahr
5 6 was one of the first to report a large variation in CCT within a normal population, and studies by Ehlers et al.
7 in the 1970s revealed that this variation in CCT had an effect on applanation measured IOP. Many studies since have been focused on the influence of CCT on IOP measurement, and although some have found no association,
8 9 most have agreed with the findings of Ehlers et al.
7 that there is an increase in measured IOP with increasing CCT
10 11 12 and that all commonly used types of tonometer are affected.
13 However, CCT alone accounts for only a small proportion of the variation in measured IOP among individuals, and it is likely that other corneal biomechanical properties have an important influence on IOP measurement.
Reichert has produced an instrument, the Ocular Response Analyzer (ORA; Reichert, Corp., Buffalo, NY), that measures the corneal response to indentation by a rapid air pulse. A feature of the ORA is that the air-jet pressure is steadily increased until the first force-in applanation event is reached. This applanation event is the trigger to switch off the air jet after some further increase in air pressure. After reaching the pressure peak, the air pressure slowly reduces until it is completely removed. The instrument makes two measurements of the corneal response to the air pressure pulse—the force necessary to flatten the cornea as the air pressure rises and the force at which the cornea flattens again as the air pressure falls.
14 It has been found that the second, force-out applanation occurs at a lower pressure than the first, force-in applanation, and this effect has been attributed to the dampening effects of the cornea. The difference between the two pressures has been termed
corneal hysteresis (CH;
Fig. 1 ). Hysteresis is a measure of the energy absorption during the “loading–unloading, stress–strain cycle of viscoelastic materials (see Fig. A1, Appendix). CH is a direct measure of the corneal biomechanical properties and therefore may more completely describe the contribution of corneal resistance to IOP measurements than does CCT alone. The contribution of the anterior ocular structures to the observed ocular hysteresis under ORA pressure is likely to be small due to the direct pressure application on the cornea. For this reason, we refer to hysteresis as a corneal response in this article.
The primary purpose of this study was to investigate the effect of IOP on CH, with a view to using CH measurements to normalize the ORA IOP measurements for the effect of corneal biomechanical properties. The secondary aims were to investigate the association between CH values and both CCT and age. Previous work has suggested that increasing age has an effect on IOP measurements,
15 and we wanted to determine whether age has any effect on the material properties of the cornea. The final purpose was to investigate the agreement between ORA IOP and GAT IOP measurements.
Study I: The Associations of CH.
Study II: Agreement between Measuring Devices.
A change in GAT IOP and P1 and P2 was observed in eyes treated with apraclonidine, as well as in control eyes, and in subsequent analyses, both treated and control eyes were assessed together. There was a change in CH (arbitrary units) for every 1-mm Hg change in GAT IOP of −1.7 (eyes treated with apraclonidine), −1.8 (eyes treated with placebo), and −1.6 (both eyes together). This illustrates that CH is dependent on IOP, although the relationship is relatively weak.
The coefficients of variation of CCT (μm), GAT IOP (mm Hg), and ORA P1 and P2 (arbitrary units) measurements are tabulated
(Table 3) . An orthogonal regression was performed to evaluate the relationship between ΔP1 and ΔP2
(Fig. 2) . There was no association between the residuals from the regression and GAT IOP or CCT.
The orthogonal regression analysis showed that P1 and P2 were linearly related, and the equations generated from this analysis were
\[{\Delta}\mathrm{P}2{=}{-}6.70{+}(1.3{\cdot}{\Delta}\mathrm{P}1),\]
for apraclonidine treated eyes,
\[{\Delta}\mathrm{P}2{=}{-}3.50{+}(1.3{\cdot}{\Delta}\mathrm{P}1),\]
for control eyes, and
\[{\Delta}\mathrm{P}2{=}{-}3.98{+}(1.27{\cdot}{\Delta}\mathrm{P}1),\]
for both eyes together.
Because of the small value of the constant in the
equation 3 , the relationship between ΔP1 and ΔP2 may be approximated by
\[{\Delta}\mathrm{P}2{=}1.27{\cdot}{\Delta}\mathrm{P}1\]
or
\[{\Delta}\mathrm{P}1{=}0.79{\cdot}{\Delta}\mathrm{P}2.\]
The calculations demonstrate that for every unit change in P1 resulting from a change in IOP, there is a corresponding, proportional change in P2, accounting for the finding that CH (P1 − P2) changes as IOP changes
(Fig. 3) . To obtain a corneal factor that does not change with IOP (CCF), we used a constant
k to adjust P2 with the coefficient derived from
equation 4(i.e., 0.79), so that:
\[\mathrm{CCF}{=}\mathrm{P}1{-}k\mathrm{P}2,\]
where
k = 0.79.
To evaluate the association of (P1 −
kP2) with CCT, various values of
k were explored, including 1 (i.e., raw P1 and P2 data output from the ORA), 0.79 (determined from the character-ization study) and other values of
k down to 0.5. A plot of the coefficient of determination for the relationship between CCT and (P1 −
kP2) for various values of
k is given in
Figure 4 . For
k = 0.79,
r 2 = 0.34. The strongest association between (P1 −
kP2) and CCT (
r 2 = 0.39) was found with
k = 0.68.
The second step was to convert the raw applanation units into pressure units (mm Hg), and a regression analysis was performed to evaluate the relationship between ΔP1 and ΔGAT
(Fig. 5) .
The relationship between ΔP1 and ΔGAT was found to be linear, and the equations generated by the analysis were
\[{\Delta}\mathrm{P}1{=}(5.85{\cdot}{\Delta}\mathrm{GAT}){+}11.85,\]
for apraclonidine treated eyes,
\[{\Delta}\mathrm{P}1{=}(6.18{\cdot}{\Delta}\mathrm{GAT}){+}5.93,\]
for control eyes, and
\[{\Delta}\mathrm{P}1{=}(6.41{\cdot}{\Delta}\mathrm{GAT}){+}7.85.\]
for both eyes together.
Due to the small value of the constant in the equations, the relationship between ΔP1 and ΔGAT may be approximated as
\[{\Delta}\mathrm{P}1{=}6.41{\cdot}{\Delta}\mathrm{GAT}.\]
From
equations 4and
6 , P1 and P2 (arbitrary units) were converted into GAT equivalents (mm Hg), and the corneal constant factor (CCF; mm Hg) was defined by
\[\mathrm{P}1_{\mathrm{mm\ Hg}}{=}\mathrm{P}1_{\mathrm{arbitrary\ units}}/6.41\]
\[\mathrm{P}2_{\mathrm{mm\ Hg}}{=}\mathrm{P}2_{\mathrm{arbitrary\ units}}/6.41{\cdot}0.79\]
and
\[\mathrm{CCF}_{\mathrm{mm\ Hg}}{=}\mathrm{P}1_{\mathrm{mm\ Hg}}{-}\mathrm{P}2_{\mathrm{mm\ Hg}}.\]
To assess the independence of CCF (mm Hg) from IOP, these calculations were applied to the characterization dataset, and the difference between CCF at baseline and after 3 hours (ΔCCF) was determined. The relationship between ΔCCF and ΔGAT confirmed the IOP independence of CCF (slope 0.013, 95% confidence interval [CI] for slope −0.04 to 0.04; r 2 = 0.001, P = 0.64).
A second corneal factor was defined using
k = 0.68, which resulted in the strongest association between CCT and (P1 –
kP2), and was termed the corneal resistance factor (CRF):
\[\mathrm{P}1_{\mathrm{mm\ Hg}}{=}\mathrm{P}1_{\mathrm{arbitrary\ units}}/6.41,\]
\[\mathrm{P}2_{\mathrm{mm\ Hg}}{=}\mathrm{P}2_{\mathrm{arbitrary\ units}}/6.41{\cdot}0.68,\]
\[\mathrm{CRF}_{\mathrm{mm\ Hg}}{=}\mathrm{P}1_{\mathrm{mm\ Hg}}{-}\mathrm{P}2_{\mathrm{mm\ Hg}}.\]
This factor, derived using
k = 0.68, is similar to that defined by the manufacturer (Luce D, personal communication, 2005):
\[\mathrm{CRF}_{\mathrm{mm\ Hg\ (Reichert)}}{=}{\{}0.1324{[}(\mathrm{P}1{-}0.7)\mathrm{P}2{]}{\}}{-}7.46\]
\[\mathrm{CRF}_{\mathrm{mm\ Hg\ (present\ study)}}{=}0.1560{[}(\mathrm{P1{-}0.68)P}2{]}.\]
where P1 and P2 are in arbitrary units.
Having determined the corneal constant factor, we wanted to explore the relationship between P1, P2, and CCF with CCT, to determine a method of removing the corneal effect from the IOP measurement
(Fig. 6) .
The relationship between CCT and P1, P2, and CCF were found to be linear, and the equations generated from the analysis were as follows:
\[\mathrm{P}1{=}(0.091{\cdot}\mathrm{CCT}){-}5.0;\ r^{2}{=}0.23,\]
\[\mathrm{P}2{=}(0.056{\cdot}\mathrm{CCT}){-}6.0;\ r^{2}{=}0.12,\]
\[\mathrm{CCF}{=}(0.033{\cdot}\mathrm{CCT}){+}1.0;\ r^{2}{=}0.34.\]
CCF was moderately strongly associated with CCT. As P2 appeared to be less affected by CCT than P1 and to a similar extent as the CCF, we hypothesized that subtracting this corneal factor from P2 would reduce the CCT dependence of P2 measurements.
Therefore, “normalized” ORA IOP was defined as
\[\mathrm{ORA\ IOP}_{\mathrm{(CCF)}}{=}\mathrm{P}2_{\mathrm{mm\ Hg}}{-}\mathrm{CCF}_{\mathrm{mm\ Hg}}{+}\mathrm{constant}.\]
The mean difference between GAT IOP measurements and ORA IOP measurements was used to determine k, to adjust the ORA IOP measurement so that the mean difference between the two techniques was 0. The constant was 11 mm Hg. These characterizations were then applied to the validation group.
Study I: Associations of Corneal Factors.
Study II: Agreement between Measuring Devices.
For the following analyses, agreement between ORA IOP
(CCF) and GAT IOP measurements were determined. In addition, the manufacturer’s value for corneal compensated ORA IOP was determined from the following formulas provided by Reichert (D. Luce, personal communication, October 2005), and was termed ORA IOP
(CC):
\[\mathrm{ORA\ IOP}_{\mathrm{(CC)\ mm\ Hg}}{=}{\{}0.202{[}\mathrm{P}2{-}(0.430{\cdot}\mathrm{P}1){]}{\}}{+}2.05.\]
For comparison,
equation 19which defines ORA IOP
(CCF) can be rewritten as
\[\mathrm{ORA\ IOP}_{\mathrm{(CCF)\ mm\ Hg}}{=}(\mathrm{P}2{\cdot}0.79){-}{[}\mathrm{P}1{-}(0.79{\cdot}\mathrm{P}2){]}/6.41{+}11\]
or
\[\mathrm{ORA\ IOP}_{\mathrm{(CCF)\ mm\ Hg}}{=}{\{}0.246{[}\mathrm{P}2{-}(0.633{\cdot}\mathrm{P}1){]}{\}}{+}11.0,\]
where P1 and P2 are in arbitrary units, as in
equation 20 .
The coefficients of variation for IOP measurements are shown in
Table 6 .
Linear regression analysis indicated that ORA IOP
(CCF) measurements were not associated with CCT (slope 0.002, 95% CI for slope −0.02 to +0.01;
r 2 = 0.0,
P = 0.85). There was a nonsignificant trend of association between ORA IOP
(CC) and CCT (slope 0.02, 95% CI for slope −0.003 to +0.03;
r 2 = 0.02,
P = 0.09). GAT IOP measurements were significantly associated with CCT (slope 0.03, 95% CI for slope 0.01 to 0.05;
r 2 = 0.06,
P = 0.002), and more strongly associated with corneal factors (
Tables 5 7 ;
Fig. 8 ).
Agreement between GAT and ORA IOP was poor and is tabulated in
Table 8 . There was a significant relationship between GAT IOP/ORA IOP
(CCF), GAT IOP/ORA IOP
(CC), and ORA IOP
(CCF)/ORA IOP
(CC) differences and mean IOP. GAT overestimates both ORA IOP
(CCF) and ORA IOP
(CC) measurements at low IOP and underestimates them at higher IOP. In addition, ORA IOP
(CCF) underestimated ORA IOP
(CC) with increasing IOP
(Fig. 9) .
Linear regression analysis indicated that GAT IOP/ORA IOP(CC) differences were not associated with CCT, but were positively associated with CCF, indicating that GAT overestimates ORA IOP(CC), with increasing CCF. The lack of association between GAT IOP/ORA IOP(CC) differences and CCT may be explained by the positive, though nonsignificant, association between ORA IOP(CC) and CCT, which was similar to the association between GAT IOP and CCT.
GAT IOP/ORA IOP(CCF) differences, however, were associated with both corneal parameters, showing that with increasing CCT and CCF, GAT IOP overestimates ORA IOP(CCF). ORA IOP(CCF)/ORA IOP(CC) differences were also associated with both CCT and CRF, so that ORA IOP(CCF) underestimates ORA IOP(CC) with increasing CCT and CCF. These two relationships suggest that the ORA IOP(CCF) measurement is less affected by corneal material properties.
The Reichert ORA measures the corneal response to indentation by a rapid jet of air, giving a measure of the corneal biomechanical properties, termed CH. In this study, we determined that CH (P1 −
kP2, where
k = 1) varies with IOP
(Fig. 2) . This IOP dependence may be physiological or reflect an artifact of the instrument measurement, or both. The finding that (P1 − kP2) becomes independent of IOP when
k = 0.79 and that the strongest association between (P1 −
kP2) and CCT was achieved with
k = 0.68, suggests that the IOP dependence of CH may be in part related to the instrument measurement technique.
A feature of the instrumentation is that the maximum air pressure (P
max) applied to the cornea varies between subjects, and is dependent on P1, which is in turn determined by both the true IOP and the structural resistance of the individual eye, which may contribute to the observed CH change with IOP change. As the cornea is a viscoelastic material, another instrument-related factor that may influence the corneal material property at different IOP levels is the rate of application of air jet pressure to the cornea. The rate of application of force increases with P1, and this may affect the hysteresis. We attempted to isolate the instrument-related corneal response from the structurally related response by modulating the IOP within eyes and measuring the change in P1 and P2 recorded. With this method, we took an empiric approach to recalculating the IOP measurement made by the ORA, and by doing so have significantly reduced the corneal effect on measured IOP: our normalized ORA IOP
(CCF) is independent of CCT
(Fig. 8) , whereas P1 is strongly related to CCT
(Fig. 6) .
The results of both treated and control eyes were analyzed together, as the regression coefficients found when analyzing each group separately were very similar and using both eyes increased the range of values (because the IOP in one eye had not been artificially modulated), adding robustness to the analysis. The method used to characterize the ORA and convert arbitrary pressure units to mm Hg makes the assumption that any IOP-related change in biomechanics will have the same effect on both GAT and ORA measurements. When exploring the relationship between ΔP1 and ΔGAT, we was found that the regression line did not pass through 0, illustrating that a small change in P1 occurs when there is no change in GAT IOP
(Fig. 5) . The GAT measure is a static measure, whereas the P1 and P2 generated by the ORA are dynamic measures. The former is affected by the elastic properties of the cornea, and the latter is affected by both the elastic and viscous properties. In our study, there was a 3-hour interval between measurements while waiting for the apraclonidine to take effect. It is possible that in conjunction with IOP changes, corneal hydration changes may have occurred during that period which in turn may alter the elastic and viscous properties of the cornea in different proportions, as is suggested by the data in
Figure 5 . It is also possible that these corneal properties vary diurnally; the relationship between diurnal GAT and ORA (P1 and P2) measurements should be explored and is the subject of a further investigation.
The relationship between ΔP1 and ΔP2 determined that for each eye there was a corneal factor that did not change with a change in IOP (
equations 3 , 4, 5). We defined this factor as the CCF. We also defined a factor, the CRF, that is determined by finding the factor that has the strongest association with CCT.
The formulas determined in our characterization study were applied to a second dataset from untreated individuals, and the relationships of both corneal factors (CCF and CRF) showed a strong association with CCT, although the latter is to be expected, as it was designed to be so. The CCF increased 0.36 mm Hg per 10 μm CCT, indicating that thicker corneas have greater dampening properties. CCF also decreased by approximately 0.28 mm Hg per decade. Experimental ex vivo studies have shown an age-related change in corneal collagen fibril properties that may contribute to an increased stiffness of the cornea with age,
17 18 and in vivo endothelial specular microscopic studies have demonstrated corneal signs that indicate an increased corneal stiffness with age.
19 The CCF is a measure of the corneal material properties, which include both stiffness and viscoelasticity. The observed negative association between corneal viscoelastic properties with advancing age may be further evidence of an increase in cross-linkage of collagen fibrils within the cornea, making it a stiffer and less viscoelastic structure.
However, ageing changes in this study cohort should be interpreted cautiously. Normal ageing changes may be confounded by pathologic corneal biomechanical changes associated with ocular hypertension (23% of our cohort), possible glaucoma, and pigment dispersion syndrome (44% of our cohort). Viscoelastic materials exhibited both creep and stress relaxation and, as a biological material, the cornea may remodel in response to raised IOP. This in turn may have an effect on the hysteresis. Studying a normal ageing population may yield different results.
Using the CCF, we redefined the ORA IOP measurement, and assessed the relationships between this measurement (ORA IOP
(CCF)), the ORA IOP measurement determined from the manufacturer’s calibration (ORA IOP
(CC)), and GAT IOP measurements with CCT. The relationship between GAT IOP measurements and CCT was in agreement with previous findings. Our study showed an increase of 0.30 mm Hg per 10-μm increase CCT, whereas a previous study has reported slopes of 0.19 mm Hg to 0.37 mm Hg per 10-μm increase in CCT in different populations.
13 However, although the association is statistically significant, the
r 2 value indicates that only 6% of the measured variation is explained by CCT. A greater proportion of between-subject variation in GAT IOP (13%) was explained by the CCF. GAT IOP measurements increased with increasing CCF by 1 mm Hg per 0.65-mm Hg increase in CCF. An important point to note, however, is that GAT measurements are primarily affected by the elastic properties of the cornea and that ORA measures the viscoelastic response of the cornea (and potentially anterior segment) to rapid deformation. It is uncertain to what extent the elastic and viscous properties are covariates. For instance, although both GAT IOP and CH increased with increased CCT, CH declined with both higher IOP and greater age, and both these factors are likely to increase corneal stiffness.
Agreement between GAT, ORA
(CCF), and ORA
(CC) measurements was poor. The differences between devices were associated with CCT and CCF
(Table 9) . It has been established that the GAT will overestimate IOP in patients with thick corneas and underestimate IOP in those with thin corneas.
10 11 12 GAT IOP/ORA IOP
(CCF) differences increased with both increasing CCF and CCT, suggesting that in eyes with thicker and stiffer corneas, the GAT IOP measurement overestimates the ORA IOP
(CCF) measurement. GAT IOP/ORA IOP
(CC) measurement differences were not significantly associated with CCT, suggesting that the corneal biomechanical effect is not completely eliminated from the manufacturer’s ORA IOP
(CC) measurement. This is supported by the finding that the differences between ORA IOP
(CCF) and ORA IOP
(CC) measurements are negatively associated with both CCT and CCF, indicating that ORA IOP
(CC) overestimates our calibration (ORA IOP
(CCF)) in patients with thicker and stiffer corneas.
The coefficient of variation of ORA IOP measurements, however, was four to five times greater than that of GAT IOP measurements, which is similar to a previous report of the repeatability of noncontact tonometer devices.
20 Noncontact devices measure the IOP within 1 to 3 ms, approximately one-five hundredth of the cardiac cycle. As a result, the ocular pulse becomes a significant source of variability, with repeated readings producing a range of between 1 and 3 mm Hg.
21 In addition, the present study used P2 to derive IOP, and this pressure was more variable than P1
(Table 3) . ORA IOP
(CCF) measurements may be more accurate in comparison with GAT IOP measurements, but measurement variability may result in reduced precision in comparison with GAT. However, averaging repeated IOP measurements may mitigate the variability of single measurements to some extent. An additional possible reason for high variability may be that the ORA used in this study was a prototype unit. Further studies assessing the repeatability of IOP measurements on production ORA units are needed.
The approach taken in this study was to use empiric data to adjust for IOP-related changes in the measured CH and to establish the relation between CH and CCT and the subsequent calculation of a normalized IOP. However, the relation between the corneal factor CCT, measured IOP, and true IOP should be confirmed by an in vivo manometry study, and such a study is under way. These data will be used to develop a finite element model of the ORA IOP measurement process so that IOP measurement errors may be predicted in a wide range of clinical circumstances.
In summary, we have presented a technique that reduces the corneal biomechanical effect on ORA IOP measurement and defines a term, the CCF, that is moderately strongly associated with CCT and explains more of the interindividual variation in GAT IOP than does CCT. The normalized ORA IOP was independent of CCT and CCF and seems to better reduce the corneal effect from the IOP measurement than the manufacturer’s calibration. Further study is needed to establish whether this measure of corneal viscoelasticity may be influenced by globe biomechanics.
The air jet is switched off at the first applanation signal. The ORA software records the air-jet pressure at first (P1) and second (P2) applanation and at maximum pressure (P
max). The level of P
max is not constant and increases with increasing P1 (slope 1.20, 95% CI for slope 1.14–1.22,
r 2 = 0.90,
P < 0.0001). This variation in P
max has implications for the corneal loading and unloading cycle
(Figure A1).
Figure A1 shows actual strip extensiometry data from porcine corneas tested with a 5-mm/min strain rate of up to 1.0, 1.5, and 2.0 N/mm
2. The loading and unloading cycles for each specimen show that the unloading stiffness is almost constant regardless of both loading stiffness and the load level. The area between the curves, or hysteresis, however, changes.
The rate of air-jet application is nonlinear, and it is also possible to determine the rate of air-jet pressure change from the software output. Using custom software written in ANSI standard C, we extracted the slope of the air-jet pressure curve during each applanation peak. Plotting the rate of application (arbitrary units/millisecond) for various levels of P1 revealed a linear relationship (slope 0.010, 95% CI for slope 0.009–0.011, r 2 = 0.91, P < 0.0001). As the cornea is viscoelastic, the more rapid applanation at higher pressure may result in a relative stiffening of the cornea and a change in CH.
Our calculations suggest that IOP-related changes CH recorded by the ORA may be as a result of instrument-related variables. Figure A1 shows that it is also related to material behavior.
Supported by the Special Trustees of Moorfields Eye Hospital. The Ocular Response Analyzer was made available for this research by the Reichert Corporation, Buffalo, NY.
Submitted for publication May 19, 2006; revised August 2 and 15, 2006; accepted October 9, 2006.
Disclosure:
A. Kotecha, Reichert Corp. (F);
A. Elsheikh, None;
C.R. Roberts, Reichert Corp. (F);
H.G. Zhu, None;
D.F. Garway-Heath, Reichert Corp. (F)
The publication costs of this article were defrayed in part by page charge payment. This article must therefore be marked “
advertisement” in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Corresponding author: David F. Garway-Heath, Glaucoma Research Unit, Moorfields Eye Hospital, 162 City Road, London, EC1V 2PD, UK;
[email protected].
Table 1. Demographic Data of Characterization Group
Table 1. Demographic Data of Characterization Group
| Mean | SD | Range |
Eye (n, left) | 50/105 | | |
Sex (n, females) | 47/105 | | |
Age (y) | 59.9 | 13.8 | 25.5–81.8 |
CCT apraclonidine eye (μm) | 564.8 | 36.2 | 482–653 |
CCT control eye (μm) | 564.6 | 36.8 | 478–655 |
Mean keratometry apraclonidine eye (mm) | 7.7 | 0.23 | 7.2–8.3 |
Mean keratometry control eye (mm) | 7.7 | 0.24 | 7.1–8.3 |
Baseline GAT IOP apraclonidine eye (mm Hg) | 17.8 | 4.5 | 9–31 |
Baseline GAT IOP control eye (mm Hg) | 17.8 | 4.5 | 9–29 |
Change/reduction GAT IOP apraclonidine eye (mm Hg) | 5.8 | 3.0 | −0.5–12.5 |
Change/reduction GAT IOP control eye (mm Hg) | 1.2 | 1.9 | −2.5–7 |
Table 2. Demographic Data of Validation Group
Table 2. Demographic Data of Validation Group
| Mean | SD | Range |
Age (y) | 57.7 | 15.1 | 19.4–83.4 |
CCT (μm) | 553.0 | 36.6 | 464.1–634.3 |
GAT IOP (mm Hg) | 17.3 | 4.1 | 9.5–28.0 |
Diagnosis (n) | | | |
Control | 48 | | |
Ocular hypertension | 33 | | |
Pigment dispersion syndrome | 17 | | |
Suspect normal tension glaucoma | 38 | | |
Suspect primary open angle glaucoma | 8 | | |
Table 3. Repeatability of CCT and IOP Measurements Made with GAT and ORA P1 and P2
Table 3. Repeatability of CCT and IOP Measurements Made with GAT and ORA P1 and P2
CCT CoV (%) | GAT CoV (%) | P1 CoV (%) | P2 CoV (%) |
0.6 | 2.1 | 3.5 | 6.0 |
Table 4. Multiple Regression Analysis of CCT and Age against the Corneal Factors
Table 4. Multiple Regression Analysis of CCT and Age against the Corneal Factors
| CCT Coefficient | P for CCT | Age Coefficient | P for Age | Adjusted r 2 |
CH (k = 1.0) (arbitrary units) | 0.18 | <0.0001 | −0.28 | 0.0002 | 0.23 |
CCF (k = 0.79) (mm Hg) | 0.036 | <0.0001 | −0.028 | 0.007 | 0.34 |
CRF (k = 0.68) (mm Hg) | 0.041 | <0.0001 | −0.020 | 0.09 | 0.33 |
Table 5. Multiple Regression Analysis of Corneal Factors and Age against GAT IOP Measurements
Table 5. Multiple Regression Analysis of Corneal Factors and Age against GAT IOP Measurements
| Corneal Factor Coefficient | P for Corneal Factor | Age Coefficient | P for Age | Adjusted r 2 |
CH (k = 1.0) (arbitrary units) | −0.037 | 0.122 | 0.032 | 0.16 | 0.02 |
CCF (k = 0.79) (mm Hg) | 0.679 | <0.0001 | 0.047 | 0.02 | 0.15 |
CRF (k = 0.68) (mm Hg) | 0.911 | <0.0001 | 0.041 | 0.03 | 0.34 |
Table 6. Repeatability of IOP Measurements Made with GAT and ORA
Table 6. Repeatability of IOP Measurements Made with GAT and ORA
GAT IOP CoV (%) | ORA IOP(CCF) CoV (%) | ORA IOP(CC) CoV (%) | CH CoV (%) | CCF CoV (%) | CRF CoV (%) | CRF(M) CoV (%) |
2.1 | 10.9 | 8.9 | 17.5 | 11.5 | 10.5 | 18.0 |
Table 7. Linear Regression Analysis of CCT and Corneal Factors against GAT IOP Measurements
Table 7. Linear Regression Analysis of CCT and Corneal Factors against GAT IOP Measurements
| Coefficient | r 2 | P |
CCT (μm) | 0.03 | 0.06 | 0.002 |
CH (k = 1.0) (arbitrary units) | −0.04 | 0.02 | 0.062 |
CCF (k = 0.79) (mm Hg) | 0.65 | 0.13 | <0.0001 |
CRF (k = 0.68) (mm Hg) | 0.91 | 0.32 | <0.0001 |
Table 8. Agreement between Measuring Devices
Table 8. Agreement between Measuring Devices
IOP Differences | Mean Difference (mm Hg) | Limits of Agreement (mm Hg) | Slope | r 2 | P |
GAT − ORA(CCF) | 0.1 | −6.6 to 6.8 | −0.22 | 0.07 | 0.001 |
GAT − ORA(CC) | −1.7 | −7.6 to 4.3 | −0.28 | 0.17 | <0.0001 |
ORA(CCF) − ORA(CC) | −1.8 | −4.1 to 0.5 | −0.07 | 0.08 | 0.0007 |
Table 9. Linear Regression Analysis of Effect of CCT and CCF on IOP Differences between Measuring Devices
Table 9. Linear Regression Analysis of Effect of CCT and CCF on IOP Differences between Measuring Devices
IOP Differences | CCT Coefficient | r 2 | P | CCF Coefficient | r 2 | P |
GAT − ORA(CCF) | 0.026 | 0.08 | 0.0006 | 1.03 | 0.46 | <0.0001 |
GAT − ORA(CC) | 0.008 | 0.01 | 0.23 | 0.45 | 0.13 | <0.0001 |
ORA(CCF) − ORA(CC) | −0.018 | 0.32 | <0.0001 | −0.50 | 0.89 | <0.0001 |
The authors thank Ashraf M. Mahmoud for software development and technical support.
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