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Eiko Tsuiki, Akikazu Fujita, Yuki Ohsaki, Jinglei Cheng, Toshiaki Irie, Kiwamu Yoshikawa, Haruki Senoo, Kazuaki Mishima, Takashi Kitaoka, Toyoshi Fujimoto; All-trans-Retinol Generated by Rhodopsin Photobleaching Induces Rapid Recruitment of TIP47 to Lipid Droplets in the Retinal Pigment Epithelium. Invest. Ophthalmol. Vis. Sci. 2007;48(6):2858-2867. doi: 10.1167/iovs.06-0768.
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purpose. To investigate the effect of light stimulation on lipid droplets (LDs) and LD proteins in the retinal pigment epithelium (RPE).
methods. Dark-adapted mouse eyes were exposed to intense flashes of light, and ARPE-19 cells were treated with all-trans-retinol. The two specimens were labeled with BODIPY493/503 for LDs and with antibodies for three LD proteins: adipocyte differentiation-related protein (ADRP), TIP47, and Rab18. The labeling intensity in fluorescence microscopy was quantified by image analysis. Localization of mutated TIP47 was also examined. Immunoelectron microscopy was performed for ADRP in mouse RPE. Expression of TIP47 in ARPE-19 cells was knocked down by RNA interference (RNAi), and its effect on retinyl ester storage was measured by HPLC.
results. Both flashes of light on mouse eyes and all-trans-retinol on ARPE-19 cells caused rapid translocation of TIP47 from the cytosol to LDs, whereas ADRP distributed constitutively in LDs. The density of LDs did not show visible changes by any treatment. The localization of TIP47 to LDs was abolished when either the amino-terminal or the carboxyl-terminal half of the molecule was deleted, but was enhanced by a short deletion in the carboxyl terminus. Manipulation of TIP47 expression by RNAi or cDNA transfection did not affect the retinyl ester amounts in ARPE-19 cells significantly.
conclusions. All-trans-retinol generated by photobleaching in the retina induces rapid translocation of TIP47 to LDs in the RPE.
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