August 1968
Volume 7, Issue 4
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Articles  |   August 1968
Rabbit Tear Proteins. I. Detection and Quantitation of Lysozyme in Nonstimulated Tears
Author Affiliations
  • BENJAMIN BONAVIDA
    Department of Bacteriology, University of California Los Angeles, Calif. 90024.
  • ALFRED T. SAPSE
    Department of Bacteriology, University of California Los Angeles, Calif. 90024.
  • ELI E. SERCARZ
    Department of Bacteriology, University of California Los Angeles, Calif. 90024.
Investigative Ophthalmology & Visual Science August 1968, Vol.7, 435-440. doi:
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      BENJAMIN BONAVIDA, ALFRED T. SAPSE, ELI E. SERCARZ; Rabbit Tear Proteins. I. Detection and Quantitation of Lysozyme in Nonstimulated Tears. Invest. Ophthalmol. Vis. Sci. 1968;7(4):435-440.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Rabbit tear lysozyme was detected in low concentration in rabbit tears collected without any stimulation. Quantitation of rabbit tear lysozyme was found possible through the use of sensitive techniques that measure its specific enzymic activity on the bacterial cell walls of Micrococcus lysodeikticus. Rabbit tear lysozyme has an electrophoretic mobility identical to that of hen egg-white lysozyme on cellulose and acrylamide gel. The experimental use of rabbits for tear lysozyme studies is discussed.

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