February 1977
Volume 16, Issue 2
Articles  |   February 1977
Stability of mammalian lens phosphofructokinase.
Investigative Ophthalmology & Visual Science February 1977, Vol.16, 126-134. doi:
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      H M Cheng, L T Chylack, J Chien, E C Barañano; Stability of mammalian lens phosphofructokinase.. Invest. Ophthalmol. Vis. Sci. 1977;16(2):126-134.

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      © ARVO (1962-2015); The Authors (2016-present)

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Two interconvertible phosphofructokinase (PFK) forms were found in rat and human lenses; whereas only one predominant form was found in calf lens. PFK isolated from these lenses possessed a common property, i.e., pH-dependent cold (or acid) lability. The inactivation was prevented by including either adenosine triphosphate (ATP) or fructose-6-phosphate (fru-6-P) in the incubating media. The protective effect of ATP or fru-6-P was complete in rat or calf lenses. In human lens, although fru-6-P was fully protective, ATP protected only partially. The inactivation could be reversed by addition of fru-6-P, but not ATP, to the incubating media at 37.5 degrees C. Lens organ culture studies showed that the depletion of lenticular ATP seemed to precipitate the loss of PFK.


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