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M I Allman, R A Harper, M Yanoff, L J Curfman, J D Cameron, B A Flaxman; Rabbit corneal epithelial cells grown in vitro without serum.. Invest. Ophthalmol. Vis. Sci. 1976;15(8):666-668.
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Primary outgrowth cultures of normal rabbit corneal epithelium can be initiated and propagated in vitro up to 6 days in serum-free medium. By the eighth day the majority of cells have ceased to divide. Epithelial cells grown without serum show DNA synthetic activity at a level comparable to control cultures grown with added serum.
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