October 1980
Volume 19, Issue 10
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Articles  |   October 1980
Selective growth of rabbit corneal epithelial cells in culture and basement membrane collagen synthesis.
Investigative Ophthalmology & Visual Science October 1980, Vol.19, 1222-1230. doi:
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      C V Sundar-Raj, I L Freeman, S I Brown; Selective growth of rabbit corneal epithelial cells in culture and basement membrane collagen synthesis.. Invest. Ophthalmol. Vis. Sci. 1980;19(10):1222-1230.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Selective isolation of rabbit corneal epithelial cells was achieved with a medium containing D-valine. Long-term cultures of the epithelial cells synthesize basement membrane-type pro-collagen doublet chains of 160,000 and 180,000 daltons and a higher-molecular-weight species at 280,000 daltons. Some of these components are disulfide-linked. These peptides gave rise to a single pepsin-resistant collagen chain of approximate molecular size of 115,000 daltons.

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