June 1980
Volume 19, Issue 6
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Articles  |   June 1980
Isolation of the plasma membrane from corneal endothelial cells.
Investigative Ophthalmology & Visual Science June 1980, Vol.19, 648-652. doi:
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      Z S Zam, J Cerda, F M Polack; Isolation of the plasma membrane from corneal endothelial cells.. Invest. Ophthalmol. Vis. Sci. 1980;19(6):648-652.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The plasma membtanes of normal rabbit endothelail cells were isolated by the use of an aqueous two-phase polymer system. The membrane fraction was identified by electron microscopy, and a fivefold to eightfold increase in the specific activity of two plasma membrane markers. Na+-K+-ATPase and 5'nucleotidase was found. Recovery of the enzyme markers averaged 45% and 22%, respectively. Analysis of the purified membranes for glucose-6-phosphatase, a marker for endoplasmic reticulum, showed no contamination by this structure. This method for cell membrane characterization is promising in determining the enzymatic alterations of diseased corneas.

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