May 1980
Volume 19, Issue 5
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Articles  |   May 1980
Thiol oxidation in the crystalline lens. I. The rate-limiting role of hexokinase in aging rat and human lenses.
Investigative Ophthalmology & Visual Science May 1980, Vol.19, 522-528. doi:
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      H M Cheng, L T Chylack; Thiol oxidation in the crystalline lens. I. The rate-limiting role of hexokinase in aging rat and human lenses.. Invest. Ophthalmol. Vis. Sci. 1980;19(5):522-528.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Reduction of nonprotein disulfides required both glucose or glucose-6-phosphate (G6P) and nicotinamide adenine dinucleotide phosphate (NADP). However, hexokinase (HK) was found to be the rate-limiting step: the glucose-supported reduction rate was only 50% of that of G6P-supported activity. This disulfide-reducing activity seemed to decline in the aging lens. Further, the glucose-supported activity dropped substantially if HK was deactivated with diamide; the deactivation was partially reversible. HK activity in aging clear and cataractous human lenses had greatly diminished. This might explain the disproportionate decrease in glucose-supported reduction in the aging lens. The components of the disulfide-reducing mechanism in human lens were reviewed and discussed.

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