August 1980
Volume 19, Issue 8
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Articles  |   August 1980
Raman spectra of normal and ultraviolet-induced cataractous rabbit lens.
Investigative Ophthalmology & Visual Science August 1980, Vol.19, 904-912. doi:
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      D M Thomas, K L Schepler; Raman spectra of normal and ultraviolet-induced cataractous rabbit lens.. Invest. Ophthalmol. Vis. Sci. 1980;19(8):904-912.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The Raman spectra of a normal rabbit lens have been recorded over the range of 0 to 4000 cm-1. Spectral results indicate that protein conformation is predominantly beta-pleated sheet. Polarization measurements and assignments for most of the bands have been made; the spectral region for water indicates extensive hydrogen bonding. An N2 laser operating at 337 nm was used to produce cataracts in a rabbit lens; the Raman spectra of the cataractous lens were also recorded. Spectral changes are observable in the water region, and these are correlated with a mechanism of ultraviolet cataractogenesis.

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