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J S Andrews, T Leonard-Martin; Total lipid and membrane lipid analysis of normal animal and human lenses.. Invest. Ophthalmol. Vis. Sci. 1981;21(1):39-45. doi: https://doi.org/.
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Comparisons of lens fiber cell membrane isolation methods were made. Although membrane lipid yield from stirring overnight in hypotonic medium followed by low-speed centrifugation was comparable to that from homogenization and high-speed centrifugation, losses in phosphatidyl ethanolamine were observed with the former method. Lyophilization with vacuum replacement by nitrogen did not exert any deleterious effects. A significant difference in total lens lipid was observed between human and the three species of animals investigated.
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