July 1981
Volume 21, Issue 1
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Articles  |   July 1981
Total lipid and membrane lipid analysis of normal animal and human lenses.
Investigative Ophthalmology & Visual Science July 1981, Vol.21, 39-45. doi:
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      J S Andrews, T Leonard-Martin; Total lipid and membrane lipid analysis of normal animal and human lenses.. Invest. Ophthalmol. Vis. Sci. 1981;21(1):39-45.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Comparisons of lens fiber cell membrane isolation methods were made. Although membrane lipid yield from stirring overnight in hypotonic medium followed by low-speed centrifugation was comparable to that from homogenization and high-speed centrifugation, losses in phosphatidyl ethanolamine were observed with the former method. Lyophilization with vacuum replacement by nitrogen did not exert any deleterious effects. A significant difference in total lens lipid was observed between human and the three species of animals investigated.

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