Purchase this article with an account.
J S Andrews, T Leonard-Martin; Total lipid and membrane lipid analysis of normal animal and human lenses.. Invest. Ophthalmol. Vis. Sci. 1981;21(1):39-45.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Comparisons of lens fiber cell membrane isolation methods were made. Although membrane lipid yield from stirring overnight in hypotonic medium followed by low-speed centrifugation was comparable to that from homogenization and high-speed centrifugation, losses in phosphatidyl ethanolamine were observed with the former method. Lyophilization with vacuum replacement by nitrogen did not exert any deleterious effects. A significant difference in total lens lipid was observed between human and the three species of animals investigated.
This PDF is available to Subscribers Only