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J S Zigler, M Mochizuki, T Kuwabara, I Gery; Purification of retinal S-antigen to homogeneity by the criterion of gel electrophoresis silver staining.. Invest. Ophthalmol. Vis. Sci. 1984;25(8):977-980.
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This report describes a procedure by which high performance liquid chromatographic (HPLC) techniques are used to obtain homogeneous S-antigen. Conventionally prepared S-antigen was purified further by fractionation on the anion exchange Mono-Q column followed by gel filtration on a TSK-3000 column. This procedure produced an S-antigen preparation, which appeared homogeneous by gel electrophoresis using the very sensitive silver staining method. The purified material retained its immunochemical and uveitogenic activity. The availability of homogeneous S-antigen will facilitate studies aimed at elucidating, at the molecular level, the mechanism by which S-antigen induces uveitis.
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