October 1984
Volume 25, Issue 10
Articles  |   October 1984
Polymorphonuclear neutrophils delay corneal epithelial wound healing in vitro.
Investigative Ophthalmology & Visual Science October 1984, Vol.25, 1217-1220. doi:
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      M D Wagoner, K R Kenyon, I K Gipson, L A Hanninen, W L Seng; Polymorphonuclear neutrophils delay corneal epithelial wound healing in vitro.. Invest. Ophthalmol. Vis. Sci. 1984;25(10):1217-1220.

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      © ARVO (1962-2015); The Authors (2016-present)

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Using an in vitro system for measuring epithelial wound healing, we studied the effect of polymorphonuclear neutrophils (PMNs) and PMN lysate on rat corneal epithelial wound healing. After 22 hr of organ culture, epithelial defects that were originally 3 mm in diameter (7.06 mm2) measured 0.41 mm2 (range, 0.17-0.72 mm2) in control rat corneas, 2.11 mm2 (range, 0.81-3.91 mm2) in corneas incubated in medium containing 5 X 10(6) PMN/ml, and 2.49 mm2 (range, 2.21-2.76 mm2) in corneas incubated with lysate obtained from an equivalent number of PMNs. Scanning and transmission electron microscopy showed similar morphology in the three groups. PMNs selectively adhered to the leading edge of the wound in corneas incubated with PMNs. These data indicate that PMNs and PMN lysate significantly slow corneal epithelial wound healing in vitro.


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