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P Russell; In vitro alterations similar to posttranslational modification of lens proteins.. Invest. Ophthalmol. Vis. Sci. 1984;25(2):209-212.
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Changes similar to posttranslational modifications that are observed with osmotic cataract formation in vivo can be seen with homogenates of lenses. These modifications are the loss of a 31,000 molecular weight (MW) beta crystallin polypeptide, an increase in a 25,000 MW membrane polypeptide. These modifications are potentiated by calcium and occur more rapidly at 37 degrees C than at 4 degrees C. Inhibitors of pepsin or of serine proteases do not influence these modifications, although proteolysis may be responsible for the changes observed.
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