July 1986
Volume 27, Issue 7
Articles  |   July 1986
Interstitial retinol-binding protein (IRBP) in subretinal fluid.
Investigative Ophthalmology & Visual Science July 1986, Vol.27, 1027-1030. doi:
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      C D Bridges, J Price, R A Landers, S L Fong, G I Liou, B S Hong, A T Tsin; Interstitial retinol-binding protein (IRBP) in subretinal fluid.. Invest. Ophthalmol. Vis. Sci. 1986;27(7):1027-1030.

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      © ARVO (1962-2015); The Authors (2016-present)

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Antibodies against bovine interstitial retinol-binding protein (b-IRBP) were used to detect human IRBP (h-IRBP) on immunoblots of eight samples of subretinal fluid (SRF) from patients with retinal detachments of between 2 days' and more than 2 years' duration. Using this sensitive technique, it was found that seven of the samples contained h-IRBP in concentrations estimated to range from below 5% up to 19% of normal human IPM. One of these samples displayed two immunoreactive bands of roughly equal intensity, one at a molecular weight of 135,000 (h-IRBP), the other at 115,000. The latter may have been generated by proteolytic cleavage. No h-IRBP could be detected in an eighth sample from a patient with retrolental fibroplasia. It is concluded that the reduced concentration of h-IRBP in SRF may be due to a number of factors that include dilution, proteolytic degradation, and metabolic inactivation of photoreceptors at the detachment site.


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