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T Shiono, S Hayasaka, K Mizuno; Delta 1-pyrroline-5-carboxylate dehydrogenase in the bovine ciliary body and iris.. Invest. Ophthalmol. Vis. Sci. 1987;28(3):459-462.
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Delta 1-pyrroline-5-carboxylate dehydrogenase extracted from the combined ciliary body and iris of bovine eyes was studied biochemically. The enzyme was purified 120-fold. The partially purified enzyme had broad optimum at pH 8.0. Apparent Km values for DL-delta 1-pyrroline-5-carboxylate and NAD were 0.14 and 0.18 mM, respectively. The enzyme activity was strongly inhibited by GABA, proline, hydroxyproline, and glutamine.
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