August 1988
Volume 29, Issue 8
Articles  |   August 1988
A scanning ocular spectrofluorophotometer.
Author Affiliations
  • J W McLaren
    Department of Ophthalmology, Mayo Clinic, Rochester, MN 55905.
  • R F Brubaker
    Department of Ophthalmology, Mayo Clinic, Rochester, MN 55905.
Investigative Ophthalmology & Visual Science August 1988, Vol.29, 1285-1293. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J W McLaren, R F Brubaker; A scanning ocular spectrofluorophotometer.. Invest. Ophthalmol. Vis. Sci. 1988;29(8):1285-1293.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements
This content is PDF only. Please click on the PDF icon to access.

We describe an instrument called a scanning ocular spectrofluorophotometer (SOSF) that measures fluorescence in a two-dimensional cross-section through the anterior chamber and cornea and provides the ability to change excitation and emission wavelengths rapidly. The output of a xenon arc lamp is filtered by a diffraction grating monochromator which has a bandpass of 4 nm and a range of 400 to 800 nm. Light emitted from the fluorophore is filtered by a variable wavelength interference filter which has a bandpass of approximately 11 nm and a range of 400 to 700 nm. To demonstrate the versatility of the instrument, we measured the spectra of fluorescein, fluorescein glucuronide and rhodamine B in the anterior chambers and corneas of pigmented rabbits after topical administration. We also measured simultaneously and independently the redistribution and disappearance of a mixture of fluorescein-labeled dextran and rhodamine B after intracameral injection. Rhodamine B was very rapidly absorbed by the cornea and lens while fluorescein-dextran was not measurable in the cornea before 4 hr. The SOSF provides a means of carrying out spectrofluorophotometry in the living eye and carrying out kinetic experiments which would otherwise be awkward or impossible.


This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.