May 1989
Volume 30, Issue 5
Free
Articles  |   May 1989
Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium.
Author Affiliations
  • H Helbig
    Institut für Klinische Physiologie, Klinikum Steglitz, Freien Universität Berlin, Federal Republic of Germany.
  • C Korbmacher
    Institut für Klinische Physiologie, Klinikum Steglitz, Freien Universität Berlin, Federal Republic of Germany.
  • J Wohlfarth
    Institut für Klinische Physiologie, Klinikum Steglitz, Freien Universität Berlin, Federal Republic of Germany.
  • M Coca-Prados
    Institut für Klinische Physiologie, Klinikum Steglitz, Freien Universität Berlin, Federal Republic of Germany.
  • M Wiederholt
    Institut für Klinische Physiologie, Klinikum Steglitz, Freien Universität Berlin, Federal Republic of Germany.
Investigative Ophthalmology & Visual Science May 1989, Vol.30, 882-889. doi:
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      H Helbig, C Korbmacher, J Wohlfarth, M Coca-Prados, M Wiederholt; Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium.. Invest. Ophthalmol. Vis. Sci. 1989;30(5):882-889.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Intracellular potentials were measured in a SV-40 virus-transformed cell clone derived from human nonpigmented ciliary epithelium using the microelectrode technique. (1) Membrane potential averaged -50.2 mV (+/- 0.6, n = 207). (2) Increasing the extracellular K+ concentration depolarized the membrane voltage. The amplitude of this potential response was reduced in the presence of 1 mM Ba2+. (3) Superfusing the cells with a Ca2+-free solution containing 1 mM EGTA depolarized the intracellular potential and diminished the voltage response upon increasing extracellular K+. (4) Extracellular alkalinization hyperpolarized the membrane potential and increased the voltage amplitude on increasing extracellular K+. (5) Addition of ouabain immediately reduced the intracellular potential. Removing extracellular K+ depolarized membrane voltage, readdition of K+ after K+ depletion transiently hyperpolarized intracellular voltage. Both potential responses were inhibited in the presence of ouabain. (6) Replacing extracellular Cl- by cyclamate resulted in a transient depolarization followed by a hyperpolarization. In the presence of SITS or DIDS (greater than or equal to 0.1 mM) the electrical responses of the cell membrane to Cl- replacement were blocked. We conclude that cultured human nonpigmented ciliary epithelial cells possess an electrogenic Na+/K+-ATPase, a K+ conductance modulated by Ca2+ and pH, and a Cl- conductance sensitive to stilbene derivatives.

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