October 1989
Volume 30, Issue 10
Free
Articles  |   October 1989
45Ca uptake by retinal pigment epithelial cells.
Author Affiliations
  • R Salceda
    Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México D.F.
Investigative Ophthalmology & Visual Science October 1989, Vol.30, 2114-2117. doi:
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      R Salceda; 45Ca uptake by retinal pigment epithelial cells.. Invest. Ophthalmol. Vis. Sci. 1989;30(10):2114-2117.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Uptake of 45Ca was studied in isolated frog retinal pigment epithelial cells. 45Ca accumulation was found to be a saturable, temperature-dependent event. Kinetic analysis of this accumulation revealed two transport systems with apparent km of 2.0 and 0.3 mM. We found the presence of a Na-Ca exchanger mechanism that releases Ca2 under depolarized conditions. Light induced an increase of 45Ca uptake due to activation of the Na-K-ATPase and consequent decrease of extracellular potassium concentration.

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