January 1989
Volume 30, Issue 1
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Articles  |   January 1989
Glucocorticoid-induced cataract in chick embryo monitored by Raman spectroscopy.
Author Affiliations
  • A Mizuno
    Department of Biochemistry, Jikei University School of Medicine, Tokyo, Japan.
  • H Nishigori
    Department of Biochemistry, Jikei University School of Medicine, Tokyo, Japan.
  • M Iwatsuru
    Department of Biochemistry, Jikei University School of Medicine, Tokyo, Japan.
Investigative Ophthalmology & Visual Science January 1989, Vol.30, 132-137. doi:
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      A Mizuno, H Nishigori, M Iwatsuru; Glucocorticoid-induced cataract in chick embryo monitored by Raman spectroscopy.. Invest. Ophthalmol. Vis. Sci. 1989;30(1):132-137.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Glucocorticoid-induced cataract lens in chick embryo was monitored by laser Raman spectroscopy. The lens opacity that appeared in chick embryo is a reversible one. Raman spectra show no significant change in the relative content of water or secondary structure of the proteins upon lens opacification. The intensity ratios of tyrosine doublet bands in Raman spectra between clear and opaque lens portions are changes. This change is reversible, and is interpreted as a protein-water phase separation that occurred during lens opacification.

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