January 1989
Volume 30, Issue 1
Free
Articles  |   January 1989
Effects of selenium on ion homeostasis and transparency in cultured lenses.
Author Affiliations
  • K R Hightower
    Eye Research Institute, Oakland University, Rochester, Michigan 48309-4401.
  • J P McCready
    Eye Research Institute, Oakland University, Rochester, Michigan 48309-4401.
Investigative Ophthalmology & Visual Science January 1989, Vol.30, 171-175. doi:
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      K R Hightower, J P McCready; Effects of selenium on ion homeostasis and transparency in cultured lenses.. Invest. Ophthalmol. Vis. Sci. 1989;30(1):171-175.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Selenium toxicity was investigated in cultured rabbit lenses to provide further information about the role of Ca++ in Se cataract. At a dose of 0.1 mM for 20 hr, Se induces a 10% change in Na levels within 6 hr, a 30% increase after 20 hr, and a three-fold increase within 48 hr of subsequent culture after removal of Se. In contrast, Ca++ levels remained normal throughout the first 24 hr. Only a small, 25% decline in GSH was noted. Not until lenses begin to swell and become noticeably opaque and turbid were Ca++ levels found to be elevated. Thus, at 72 hr, 48 hr following the removal of selenium, Ca++ had increased to a concentration of 0.7 mM. Ca++ accumulation appears to be a consequence of osmotic stress rather than pump inhibition while Na accumulation is a direct consequence of Se-inhibited Na pump.

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