August 1989
Volume 30, Issue 8
Free
Articles  |   August 1989
Neuronal differentiation of retinoblastoma cells induced by medium conditioned by human RPE cells.
Author Affiliations
  • J Tombran-Tink
    Department of Anatomy and Cell Biology, University of Southern California, Los Angeles 90033.
  • L V Johnson
    Department of Anatomy and Cell Biology, University of Southern California, Los Angeles 90033.
Investigative Ophthalmology & Visual Science August 1989, Vol.30, 1700-1707. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J Tombran-Tink, L V Johnson; Neuronal differentiation of retinoblastoma cells induced by medium conditioned by human RPE cells.. Invest. Ophthalmol. Vis. Sci. 1989;30(8):1700-1707.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Tumor cells can be induced to differentiate in vitro by biochemical manipulation of their culture environment. In the studies described here, the effects of medium conditioned by human retinal pigmented epithelial (RPE) cells on Y79 human retinoblastoma cells have been examined. RPE-conditioned medium in conjunction with laminin and a poly-D-lysine substratum is observed to induce neuronal differentiation of Y79 cells. The cells extend long cellular processes and exhibit immunologically detectable neurotypic properties. In contrast, control Y79 cells not exposed to medium conditioned by RPE cells exhibit only infrequent neuronal phenotypes. This response of Y79 cells to RPE-conditioned medium indicates that factors secreted by RPE cells can act as inducers of neuronal differentiation in retinoblastoma cells and suggest that such factors may be of importance in the development and differentiation of the neural retina.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×