July 1989
Volume 30, Issue 7
Free
Articles  |   July 1989
Oxidative inhibition of Ca2+-ATPase in the rabbit lens.
Author Affiliations
  • D Borchman
    Department of Ophthalmology, University of Louisville School of Medicine, Kentucky Lions Eye Research Institute, Kentucky 40202.
  • C A Paterson
    Department of Ophthalmology, University of Louisville School of Medicine, Kentucky Lions Eye Research Institute, Kentucky 40202.
  • N A Delamere
    Department of Ophthalmology, University of Louisville School of Medicine, Kentucky Lions Eye Research Institute, Kentucky 40202.
Investigative Ophthalmology & Visual Science July 1989, Vol.30, 1633-1637. doi:
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      D Borchman, C A Paterson, N A Delamere; Oxidative inhibition of Ca2+-ATPase in the rabbit lens.. Invest. Ophthalmol. Vis. Sci. 1989;30(7):1633-1637.

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Abstract

Hydrogen peroxide inhibition of maximum Ca2+-ATPase and Na+,K+-ATPase activity was measured in a membrane-enriched preparation of rabbit lens cortical fibers and epithelium. At 5 X 10(-6) M hydrogen peroxide maximum Ca2+-ATPase activity was inhibited by 39%, while maximum Na+,K+-ATPase activity was stimulated. Ca2+-ATPase activity was almost completely inhibited at 5 X 10(-4) M hydrogen peroxide, in comparison to Na+,K+-ATPase activity, which was only inhibited by 28% at a concentration of hydrogen peroxide an order of magnitude larger. The addition of catalase to hydrogen peroxide-pretreated samples did not reverse the inhibition of Ca2+-ATPase by hydrogen peroxide.

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