October 1989
Volume 30, Issue 10
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Articles  |   October 1989
Furosemide-sensitive Cl transport in bovine retinal pigment epithelium.
Author Affiliations
  • D A Frambach
    Department of Ophthalmology, University of Southern California, Los Angeles.
  • J L Valentine
    Department of Ophthalmology, University of Southern California, Los Angeles.
  • J J Weiter
    Department of Ophthalmology, University of Southern California, Los Angeles.
Investigative Ophthalmology & Visual Science October 1989, Vol.30, 2271-2274. doi:
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      D A Frambach, J L Valentine, J J Weiter; Furosemide-sensitive Cl transport in bovine retinal pigment epithelium.. Invest. Ophthalmol. Vis. Sci. 1989;30(10):2271-2274.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Bovine retinal pigment epithelium (RPE)-choroid explants were sealed in an Ussing chamber. Typical preparations produced a transepithelial voltage (Ve) of 12 mV (retina side positive) and had an electrical resistance (R) of 300 ohm-cm2. These values can be attributed to the RPE. Furosemide and ouabain reduced the Ve without affecting R when applied to the apical side of the RPE, but had no effect upon Ve and R when applied to the choroidal side. Acetazolamide had no effect upon Ve and R when applied to either side of the tissue. In Cl-free medium, ouabain reduced Ve without affecting R, while furosemide had no effect upon Ve and R. In Na-free medium, ouabain and furosemide had no effect upon Ve and R. Unidirectional isotope flux studies performed under open circuit conditions showed a net retina-to-choroid Cl flux that was abolished by furosemide. These results indicate that bovine RPE possesses a furosemide-sensitive Cl transport system.

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