May 1989
Volume 30, Issue 5
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Articles  |   May 1989
Experimentally induced ocular chlamydial infection in infant pig-tailed macaques.
Author Affiliations
  • P A Cosgrove
    Department of Obstetrics and Gynecology, University of Washington 98195.
  • D L Patton
    Department of Obstetrics and Gynecology, University of Washington 98195.
  • C C Kuo
    Department of Obstetrics and Gynecology, University of Washington 98195.
  • S P Wang
    Department of Obstetrics and Gynecology, University of Washington 98195.
  • T D Lindquist
    Department of Obstetrics and Gynecology, University of Washington 98195.
Investigative Ophthalmology & Visual Science May 1989, Vol.30, 995-1003. doi:
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    • Get Citation

      P A Cosgrove, D L Patton, C C Kuo, S P Wang, T D Lindquist; Experimentally induced ocular chlamydial infection in infant pig-tailed macaques.. Invest. Ophthalmol. Vis. Sci. 1989;30(5):995-1003.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Four Macaca nemestrina monkeys were inoculated in the conjunctiva with Chlamydia trachomatis (strain E) at 6 weeks of age. A fifth monkey was inoculated with HeLa cell materials only. Ten weeks later, all monkeys were reinoculated with either strain E or strain C. All inoculated monkeys were susceptible to infection with C. trachomatis as documented by fluorescent antibody staining of smears and reisolation of the organism from conjunctival and nasopharyngeal swab specimens. Rectal and vaginal swab specimens remained negative throughout the study. Three of four inoculated animals responded with IgM titers reaching a peak of 1:16 (M#3) and 1:32 (M#1, M#4) 2 weeks after the primary inoculation. IgG appeared in all inoculated animals and titers rose to peak levels of 1:64 (M#2), 1:128 (M#1, M#3), and 1:256 (M#4). Histopathology documented a dramatic difference in immunological response following secondary inoculation. Primary inoculation elicited a typical inflammatory response characterized by moderate stromal infiltration of polymorphonuclear and mononuclear leukocytes. Plasma cells appeared by week 3 postinoculation (pi). Following a secondary inoculation, classic follicle formation was evident by 1 week pi. Mononuclear markers identified a germinal center composed of B cells and a T cell cap. Epithelial thinning near the cap of the follicle was accompanied by a complete loss of goblet cells. This model may be useful for studying the immunopathology of infant chlamydial infections.

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