December 1992
Volume 33, Issue 13
Free
Articles  |   December 1992
Cytokeratin expression in corneal endothelium in the iridocorneal endothelial syndrome.
Author Affiliations
  • T R Kramer
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia.
  • H E Grossniklaus
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia.
  • N Vigneswaran
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia.
  • G O Waring
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia.
  • A Kozarsky
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia.
Investigative Ophthalmology & Visual Science December 1992, Vol.33, 3581-3585. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T R Kramer, H E Grossniklaus, N Vigneswaran, G O Waring, A Kozarsky; Cytokeratin expression in corneal endothelium in the iridocorneal endothelial syndrome.. Invest. Ophthalmol. Vis. Sci. 1992;33(13):3581-3585.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

The immunocytologic characteristics of two formalin-fixed, paraffin-embedded corneas from patients with the iridocorneal endothelial (ICE) syndrome and unaffected control corneas were studied. Binding of polyclonal antisera to Factor VIII, S-100 protein, involucrin, neuron specific enolase (NSE), and the lectins peanut agglutinin and Ulex europaeus agglutinin-1 was performed using the standard peroxidase-anti-peroxidase method. We detected reactive patterns of monoclonal antibodies to cytokeratins (34BE12 is a 56-58 kD mouse IgG reactive to stratified epithelia; Pkk1 is a 44-54 kD mouse IgG reactive to simple epithelia; and KL1 is a 55-57 kD mouse IgG reactive to epidermis and simple epithelia) using the standard avidin-biotin complex method. Staining properties were similar for the polyclonal antisera, lectins, NSE, and chromogranin in corneas with ICE syndrome and in the controls. However, the cytokeratins 34BE12, Pkk1, and KL1 were detected in the endothelium of the corneas with the ICE syndrome but not in the controls. These findings suggest that various cytokeratins are expressed in the corneal endothelium in the ICE syndrome that are not expressed in unaffected corneal endothelium.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×