November 1992
Volume 33, Issue 12
Free
Articles  |   November 1992
T cell depletion increases susceptibility to murine cytomegalovirus retinitis.
Author Affiliations
  • S S Atherton
    Department of Microbiology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida.
  • C K Newell
    Department of Microbiology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida.
  • M Y Kanter
    Department of Microbiology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida.
  • S W Cousins
    Department of Microbiology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida.
Investigative Ophthalmology & Visual Science November 1992, Vol.33, 3353-3360. doi:
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    • Get Citation

      S S Atherton, C K Newell, M Y Kanter, S W Cousins; T cell depletion increases susceptibility to murine cytomegalovirus retinitis.. Invest. Ophthalmol. Vis. Sci. 1992;33(12):3353-3360.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

To study the effect of immunosuppression on the development of murine cytomegalovirus (MCMV) retinitis, BALB/c mice were immunosuppressed with methylprednisolone (a corticosteroid) and/or with antibodies against CD4+ and CD8+ T cells and inoculated with low-dose MCMV (5 x 10(2) plaque-forming units) by the supraciliary route. Nonimmunosuppressed mice inoculated with low-dose MCMV by the supraciliary route did not develop necrotizing retinitis. By contrast, 78-100% of immunosuppressed mice developed retinitis after inoculation of low-dose MCMV. To study the effect of depletion of individual T cell subsets, mice were depleted of either CD4+ or CD8+ T cells and inoculated with low-dose MCMV by the supraciliary route. The frequency of retinitis in CD4-depleted mice (30%) was not significantly different from that of nonimmunosuppressed control mice (0%). The frequency of retinitis in the CD8-depleted group (80%) was similar to that observed in mice immunosuppressed with corticosteroid alone (90.9%), with antibodies to both T cell subsets (100%), or with steroid and both T cell subset antibodies (100%). These results support the conclusion that the CD8+ T cell subset is responsible for control of ocular MCMV infection. Furthermore, these results suggest that the CD8+ T cell subset may be important in preventing ocular CMV infection in immunosuppressed patients.

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