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S Kuriyama, T Ohuchi, N Yoshimura, Y Honda; Growth factor-induced cytosolic calcium ion transients in cultured human retinal pigment epithelial cells.. Invest. Ophthalmol. Vis. Sci. 1991;32(11):2882-2890.
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Growth factor-induced changes of cytosolic free calcium ion concentration ([Ca2+]i) and phosphatidylinositol (PI) turnover in cultured human retinal pigment epithelial (RPE) cells were studied, and their temporal relationship was compared. After a 24-hr serum depletion, RPE cells were loaded with fura-2/AM, and [Ca2+]i was analyzed using a digital imaging microscopy system. The resting [Ca2+]i in a single cultured human RPE cell was 153 +/- 1.5 nM (mean +/- the standard error of the mean [SEM], n = 105). The percentage of reactive cells that had Ca2+ transients induced by various growth factors were: epidermal growth factor, 18%; basic fibroblast growth factor (bFGF), 5%; nerve growth factor, 15%; platelet-derived growth factor (PDGF), 70%; insulin-like growth factor, 0%; fibronectin, 0%; insulin, 3%; and fetal bovine serum (FBS), 100%. The PDGF showed a peak concentration of 237 +/- 4.7 nM (mean +/- SEM, n = 67) and FBS, 774 +/- 34.5 nM (mean +/- SEM, n = 52). Chelation of the extracellular Ca2+ by EGTA made the resting and peak concentration lower. The Ca2+ transients occurred within 60 sec and lasted less than 5 min after the application of the growth factors. However, measurements of phosphoinositides in 24-hr serum-starved RPE cells revealed that growth factor-induced PI turnover was much slower than Ca2+ transients. In addition, FBS, bFGF, and PDGF increased the contents of inositol phosphate, inositol biphosphate, and inositol triphosphate (IP3) in RPE cells slowly up to 60 min except that PDGF showed a peak of IP3 at 15 min after stimulation.(ABSTRACT TRUNCATED AT 250 WORDS)
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