October 1991
Volume 32, Issue 11
Free
Articles  |   October 1991
Interferon-gamma inhibits collagen synthesis by human Tenon's capsule fibroblasts in vitro.
Author Affiliations
  • S Q Low
    Jules Stein Eye Institute, UCLA School of Medicine 90024-7004.
  • S Kitada
    Jules Stein Eye Institute, UCLA School of Medicine 90024-7004.
  • D A Lee
    Jules Stein Eye Institute, UCLA School of Medicine 90024-7004.
Investigative Ophthalmology & Visual Science October 1991, Vol.32, 2964-2969. doi:
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      S Q Low, S Kitada, D A Lee; Interferon-gamma inhibits collagen synthesis by human Tenon's capsule fibroblasts in vitro.. Invest. Ophthalmol. Vis. Sci. 1991;32(11):2964-2969.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Collagen deposition is largely responsible for scar information, an undesirable outcome of glaucoma filtering surgery. Because interferon-gamma (IFN-gamma) has been reported to inhibit collagen synthesis in a variety of cells, the authors examined its effect on collagen synthesis by Tenon's capsule fibroblasts cultured from three patients. In addition, the authors studied the effects of IFN-gamma on fibroblast proliferation. IFN-gamma inhibited collagen synthesis at dosages of 1-10,000 U/ml. The average median effective dose (ED50) was 6.60 U/ml. In contrast, IFN-gamma had no significant effect on cell proliferation in dosages of 0.001-10,000 U/ml. These results suggest that IFN-gamma may be a useful agent for modifying the wound healing response, particularly after glaucoma filtering surgery.

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