August 1992
Volume 33, Issue 9
Free
Articles  |   August 1992
Endothelial leukocyte adhesion molecule-1 in endotoxin-induced uveitis.
Author Affiliations
  • S M Whitcup
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
  • D Wakefield
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
  • Q Li
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
  • R B Nussenblatt
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
  • C C Chan
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
Investigative Ophthalmology & Visual Science August 1992, Vol.33, 2626-2630. doi:
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    • Get Citation

      S M Whitcup, D Wakefield, Q Li, R B Nussenblatt, C C Chan; Endothelial leukocyte adhesion molecule-1 in endotoxin-induced uveitis.. Invest. Ophthalmol. Vis. Sci. 1992;33(9):2626-2630.

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Abstract

Expression of endothelial leukocyte adhesion molecule-1 (ELAM-1) on endothelial cells leads to the attachment of polymorphonuclear leukocytes. The sequential expression of ELAM-1 and major histocompatibility complex (MHC) class II antigen was examined in the eyes of 59 Lewis rats with endotoxin-induced uveitis (EIU) after the injection of Salmonella typhimurium endotoxin. The eyes were enucleated at 2-hr intervals. Hematoxylin and eosin-stained paraffin-embedded sections and immunohistochemically stained cryostat sections were graded by two masked observers. The MHC class II antigen was expressed on cells in the iris and ciliary body 4 hr after injection of endotoxin and on the corneal endothelium, 8 hr postinjection. It was found that ELAM-1 was expressed first on cells of the ciliary body and iris 10 hr after the injection of endotoxin and on the corneal endothelium, 22 hr postinjection. Clinical and histopathologic disease developed 16 hr postinjection. Adherence of polymorphonuclear cells to the corneal endothelium was observed at the time of ELAM-1 expression. In conclusion, expression of ELAM-1 on ocular tissue occurred in EIU and appeared to promote polymorphonuclear cell accumulation in the anterior segment of the eye.

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