March 1993
Volume 34, Issue 3
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Articles  |   March 1993
Heterogeneity in localization of isoforms of TGF-beta in human retina, vitreous, and choroid.
Author Affiliations
  • G A Lutty
    Johns Hopkins School of Medicine, Baltimore, Maryland 21287-9115.
  • C Merges
    Johns Hopkins School of Medicine, Baltimore, Maryland 21287-9115.
  • A B Threlkeld
    Johns Hopkins School of Medicine, Baltimore, Maryland 21287-9115.
  • S Crone
    Johns Hopkins School of Medicine, Baltimore, Maryland 21287-9115.
  • D S McLeod
    Johns Hopkins School of Medicine, Baltimore, Maryland 21287-9115.
Investigative Ophthalmology & Visual Science March 1993, Vol.34, 477-487. doi:
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      G A Lutty, C Merges, A B Threlkeld, S Crone, D S McLeod; Heterogeneity in localization of isoforms of TGF-beta in human retina, vitreous, and choroid.. Invest. Ophthalmol. Vis. Sci. 1993;34(3):477-487.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: The purpose of this work was to investigate further the immunohistochemical localization of transforming growth factor (TGF)-beta 1 to photoreceptors, which we had previously observed, to elucidate the cell types of origin for TGF-beta 1, and to determine sites of localization for TGF-beta 2 and beta 3 in the posterior segment of the human eye. METHOD: Streptavidin-peroxidase immunohistochemistry was used to localize TGF-beta antibodies in sections from cryopreserved human posterior segments from six adult donors. Two recently described polyclonal antibodies to TGF-beta 1 (anti-CC[1-30] and anti-LC[1-30]) and antibodies against TGF-beta 2 and beta 3 were employed in this study. Other studies suggest that anti-LC(1-30) antibody recognizes pre-pro-TGF-beta 1 and therefore sites of production, while anti-CC(1-30) recognizes secreted forms of TGF-beta 1. RESULTS: Regional labeling of choriocapillaris was observed in all eyes using anti-LC(1-30) (anti-pre-pro-beta 1). Anti-CC(1-30) (anti-secr-beta 1) was localized predominantly in photoreceptor inner and outer segments in all eyes. Outer segment localization appeared identical to anti-opsin localization, indicating that this form of TGF-beta 1 is most likely intracellular. TGF-beta 2 was observed predominantly in the connective tissue of long ciliary arteries in choroid and in photoreceptor outer segments in all eyes. TGF-beta 3 was observed in isolated individual cells in choroid and retina. Vitreous hyalocytes were the only cell type examined that were immunoreactive for all four forms of TGF-beta studied. CONCLUSION: The heterogeneity in localization of TGF-beta s in the human posterior eye suggests that TGF-beta s are produced, used, and degraded locally. TGF-beta 1 produced by the choriocapillaris may be stored or used by photoreceptors.

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