July 1992
Volume 33, Issue 8
Free
Articles  |   July 1992
Collagen gel contraction by cells associated with proliferative vitreoretinopathy.
Author Affiliations
  • C Guidry
    Department of Biochemistry, University of Alabama, Birmingham 35294.
  • R J McFarland
    Department of Biochemistry, University of Alabama, Birmingham 35294.
  • R Morris
    Department of Biochemistry, University of Alabama, Birmingham 35294.
  • C D Witherspoon
    Department of Biochemistry, University of Alabama, Birmingham 35294.
  • M Hook
    Department of Biochemistry, University of Alabama, Birmingham 35294.
Investigative Ophthalmology & Visual Science July 1992, Vol.33, 2429-2435. doi:
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    • Get Citation

      C Guidry, R J McFarland, R Morris, C D Witherspoon, M Hook; Collagen gel contraction by cells associated with proliferative vitreoretinopathy.. Invest. Ophthalmol. Vis. Sci. 1992;33(8):2429-2435.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The capacities of porcine choroidal fibroblasts, retinal glial cells, and retinal pigment epithelial cells to contract collagen gels in vitro were compared. Experiments with varied cell numbers indicated that glial cells are the most effective, followed by choroidal fibroblasts and retinal pigment epithelial cells. Analysis of the secretory products from cultures of these cell types revealed that retinal pigment epithelial cells synthesize and secrete peptides that promote fibroblast contraction of collagen gels in vitro. The mechanism of action of the retinal pigment epithelial cell-secreted contraction promoter was compared with that found in serum (type A) and secreted by cultured endothelial cells (type B). Like the serum factor, the retinal pigment epithelial cell-secreted factor was not dependent on active protein synthesis by the target cell and must be present continuously to promote contraction.

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