February 1993
Volume 34, Issue 2
Free
Articles  |   February 1993
Retroperfusion studies of the aqueous outflow system. Part I: Evaluation of technique using N-ethyl maleimide.
Author Affiliations
  • C R Ethier
    Institute for Biomedical Engineering, University of Toronto, Ontario, Canada.
  • F M Coloma
    Institute for Biomedical Engineering, University of Toronto, Ontario, Canada.
  • A W de Kater
    Institute for Biomedical Engineering, University of Toronto, Ontario, Canada.
  • R R Allingham
    Institute for Biomedical Engineering, University of Toronto, Ontario, Canada.
Investigative Ophthalmology & Visual Science February 1993, Vol.34, 385-394. doi:https://doi.org/
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      C R Ethier, F M Coloma, A W de Kater, R R Allingham; Retroperfusion studies of the aqueous outflow system. Part I: Evaluation of technique using N-ethyl maleimide.. Invest. Ophthalmol. Vis. Sci. 1993;34(2):385-394. doi: https://doi.org/.

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Abstract

PURPOSE: The goal of this study was to develop a new technique to deliver drugs or other agents to the lumen of the angular aqueous plexus/Schlemm's canal (AAP/SC) while bypassing the trabecular meshwork, thereby gaining insight into AAP/SC inner wall function. METHODS: The anterior chamber is held at a small negative pressure and fluid is allowed to flow retrograde from the limbal vessels, through the collector channels, and into the AAP/SC ("retroperfusion"). Facility measurements are combined with histologic and tracer studies in bovine eyes. RESULTS: (1) Retroperfusion with a saline solution does not alter facility or change outflow pathway morphology; (2) fluid is able to move retrograde from the scleral surface and enter the lumen of the AAP; and (3) retroperfusion with N-ethyl maleimide causes a dose-dependent increase in washout rate and concomitant inner wall breaks. CONCLUSIONS: It is hypothesized that the observed increase in washout is due to leakage of extracellular materials through breaks in the inner wall.

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