June 1992
Volume 33, Issue 7
Articles  |   June 1992
Whole-cell currents from noncultured human lens epithelium.
Author Affiliations
  • J L Rae
    Department of Physiology, Mayo Foundation, Rochester, Minnesota 55905.
  • J S Rae
    Department of Physiology, Mayo Foundation, Rochester, Minnesota 55905.
Investigative Ophthalmology & Visual Science June 1992, Vol.33, 2262-2268. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J L Rae, J S Rae; Whole-cell currents from noncultured human lens epithelium.. Invest. Ophthalmol. Vis. Sci. 1992;33(7):2262-2268.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements
This content is PDF only. Please click on the PDF icon to access.

Perforated patch techniques were used to measure whole-cell ionic currents in freshly dissociated human lens epithelial cells that had not been subjected to culture media or serum. With a 150 mmol/l K+ internal solution, the cells had resting voltages of -27.4 +/- 4.7 mV (mean +/- standard deviation [SD]) and capacitances of 10.4 +/- 2.8 pF (mean +/- SD). The input resistance of the cells was 1.6 +/- 0.7 G omega (mean +/- SD) at large negative voltages. A delayed outwardly rectifying K+ current was found in most cells studied. Current magnitudes of 1-2 nA at +80 mV were common. The current had selectivities, activation time constants, deactivation time constants, open probability versus voltage relationships, and inactivations similar to those of the delayed rectifying K+ current found in many cell types and studied previously in cultured human lens epithelium. These results verify the existence, at high density, of these currents in noncultured human epithelial cells.


This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.