July 1992
Volume 33, Issue 8
Free
Articles  |   July 1992
Induction of elongation in cultured rat lens epithelial cells by FGF and inhibition by selenite.
Author Affiliations
  • M Azuma
    Department of Biochemistry, School of Dentistry, Oregon Health Sciences University, Portland 97201.
  • T R Shearer
    Department of Biochemistry, School of Dentistry, Oregon Health Sciences University, Portland 97201.
Investigative Ophthalmology & Visual Science July 1992, Vol.33, 2528-2531. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M Azuma, T R Shearer; Induction of elongation in cultured rat lens epithelial cells by FGF and inhibition by selenite.. Invest. Ophthalmol. Vis. Sci. 1992;33(8):2528-2531.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

The purposes of this experiment were to: (1) test if fibroblast growth factor (FGF) induced elongation of cultured rat lens epithelial cells (LEC) and (2) determine if selenite affected elongation of LEC. FGF (125-500 ng/ml) reduced the number of colonies of LEC, but it did not induce elongation when cells were cultured on plastic dishes. One hundred micromolar and, to a lesser extent, 10 mumol/l selenite also reduced the number of colonies of LEC. Coculture of FGF and selenite on plastic caused a synergistic reduction in the number of colonies. FGF (125-1000 ng/ml) induced a dramatic morphologic change in LEC. Elongated processes radiated from stellate-like cell aggregates when cells were cultured on reconstituted basement membrane matrix (Matrigel). Again, 100 mumol/l selenite and, to a lesser extent, 10 mumol/l selenite reduced the number of cell aggregates with processes on Matrigel. These results indicated that an inhibitory effect of selenite on the elongation of LEC may be a factor in the development of selenite cortical cataract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×