April 1993
Volume 34, Issue 5
Free
Articles  |   April 1993
Single-cell contraction assay for human ciliary muscle cells. Effect of carbachol.
Author Affiliations
  • I H Pang
    Glaucoma Research, Alcon Laboratories, Fort Worth, TX 76134.
  • D L Shade
    Glaucoma Research, Alcon Laboratories, Fort Worth, TX 76134.
  • E Tamm
    Glaucoma Research, Alcon Laboratories, Fort Worth, TX 76134.
  • L DeSantis
    Glaucoma Research, Alcon Laboratories, Fort Worth, TX 76134.
Investigative Ophthalmology & Visual Science April 1993, Vol.34, 1876-1879. doi:
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    • Get Citation

      I H Pang, D L Shade, E Tamm, L DeSantis; Single-cell contraction assay for human ciliary muscle cells. Effect of carbachol.. Invest. Ophthalmol. Vis. Sci. 1993;34(5):1876-1879.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: The authors developed an assay to observe the contraction of a single human ciliary muscle cell. METHODS: Cultured human ciliary muscle cells were partially detached from the culture dish by incubation with a nonenzymatic dissociation buffer and treated with carbachol or pilocarpine. Contraction was quantified by measuring the cross-sectional surface areas of the cells. RESULTS: Carbachol decreased the cell surface area in a time-dependent manner. Contraction was observed within 1 min after the addition of carbachol and completed in less than 15 min. The effect of carbachol was dose dependent. For example, at 10 min after treatment with 10 mumol/l carbachol, the relative surface areas of cells decreased to 47% +/- 4% (mean +/- standard error of the mean, n = 7, with surface area at 0 min defined as 100%). The relative surface areas were 74% +/- 4% (n = 7) after 1 mumol/l and 100% +/- 9% (n = 7) after 0.1 mumol/l carbachol treatment. This contractile effect was antagonized by pretreatment with atropine, a specific muscarinic antagonist. CONCLUSIONS: A simple method was established to study the functional changes of human ciliary muscle cells.

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