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K Hiroi, F Yamamoto, Y Honda; Analysis of electroretinogram during systemic hypercapnia with intraretinal K(+)-microelectrodes in cats.. Invest. Ophthalmol. Vis. Sci. 1994;35(11):3957-3961.
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PURPOSE: Systemic hypercapnia alters the intraretinal pH and metabolism and profoundly affects the vitreal electroretinogram (ERG). Using intraretinal K(+)-selective microelectrodes (ISMs), the authors examined the effects of systemic hypercapnia on ERG in the intact cat eye in vivo. METHODS: Double-barreled K(+)-ISMs were used to record intraretinal, light-evoked [K+]o decrease, intraretinal ERG, vitreal ERG, and standing potential (SP) in the dark-adapted retina. Hypercapnia was induced by adding CO2 to the inhaled mixture of gas (without significant hypoxia). RESULTS: The standing potential was elevated, and the vitreal ERG c-wave increased in amplitude whereas the b-wave decreased during hypercapnia. Intraretinal recordings showed that the amplitudes of slow PIII and the transepithelial (TEP) c-wave decreased. The slow PIII decreased more than the TEP c-wave. The light-evoked [K+]o decrease also was reduced, but no change was noted in the subretinal [K+]o. CONCLUSION: The neural retina was more sensitive than the retinal pigment epithelium to the intraretinal acidification caused by systemic hypercapnia. However, the change in the SP and b-wave suggested complicated effects other than acidosis.
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