October 1996
Volume 37, Issue 11
Free
Articles  |   October 1996
Visualization of leukocyte dynamics in the choroid with indocyanine green.
Author Affiliations
  • N Matsuda
    Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan.
  • Y Ogura
    Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan.
  • H Nishiwaki
    Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan.
  • K Miyamoto
    Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan.
  • T Matsubara
    Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan.
  • J Kiryu
    Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan.
  • Y Honda
    Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan.
Investigative Ophthalmology & Visual Science October 1996, Vol.37, 2228-2233. doi:
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    • Get Citation

      N Matsuda, Y Ogura, H Nishiwaki, K Miyamoto, T Matsubara, J Kiryu, Y Honda; Visualization of leukocyte dynamics in the choroid with indocyanine green.. Invest. Ophthalmol. Vis. Sci. 1996;37(11):2228-2233.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To report a method to evaluate leukocyte dynamics in the choroidal circulation with indocyanine green (ICG) angiography. METHODS: Nonpigmented and pigmented rats were administered ICG solution intravenously. The fundus image was obtained with a scanning laser ophthalmoscope and recorded on magnetic tapes at a video rate (30 frames/second). The images were analyzed with a personal computer-based image analysis system. RESULTS: On ICG angiography, hyperfluorescent dots were seen moving along the choroidal vessels and in the retinal vessels several minutes after injection. These fluorescent dots were thought to be circulating leukocytes stained with ICG. The micrographs of blood smears after ICG injection showed intense fluorescence of leukocytes. Computer-assisted image analysis allowed tracing of these fluorescent dots using a frame-by frame method. CONCLUSIONS: Results of this study indicated that ICG can be used for vital staining of leukocytes and that it is possible to evaluate leukocyte movement in the choroidal circulation in vivo in rats.

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