June 1996
Volume 37, Issue 7
Free
Articles  |   June 1996
Cone-associated c-fos gene expression in the light-damaged rat retina.
Author Affiliations
  • T Harada
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • J Imaki
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • K Ohki
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • K Ono
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • T Ohashi
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • H Matsuda
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
  • K Yoshida
    Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
Investigative Ophthalmology & Visual Science June 1996, Vol.37, 1250-1255. doi:
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    • Get Citation

      T Harada, J Imaki, K Ohki, K Ono, T Ohashi, H Matsuda, K Yoshida; Cone-associated c-fos gene expression in the light-damaged rat retina.. Invest. Ophthalmol. Vis. Sci. 1996;37(7):1250-1255.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To examine whether light-controlled c-fos gene expression is mediated by a cone specific pathway in the rat retina. METHODS: To produce a cone-rich retina, rats were placed under continuous lighting for 21 days. The illuminance in the cages ranged from 1200 to 2000 lux. The presence of cones was determined by peanut agglutinin and rods by the monoclonal antibody 1-E7. The presence of c-fos transcripts in the retinas was analyzed by in situ hybridization using single-stranded RNA probes. RESULTS: After long-term exposure to continuous light, the 1-E7 immunoreactivity was not detected in the outer nuclear layer. The cone photoreceptors and cells in the ganglion cell (GCL) and inner nuclear layers (INL) survived. In normal retinas, there was strong hybridization for c-fos expression in the GCL and the INL 30 minutes after the onset of the light cycle. Light-damaged retinas also showed hybridization in the GCL but not in the INL under the same light regime. CONCLUSIONS: These results suggest that c-fos gene expression can be controlled through a cone specific pathway in the retina.

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