May 1996
Volume 37, Issue 6
Free
Articles  |   May 1996
Temporal and spatial regulation of integrin vitronectin receptor mRNAs in the embryonic chick retina.
Author Affiliations
  • D B Gervin
    Neuroscience Research Institute, University of California, Santa Barbara 93106, USA.
  • G M Cann
    Neuroscience Research Institute, University of California, Santa Barbara 93106, USA.
  • D O Clegg
    Neuroscience Research Institute, University of California, Santa Barbara 93106, USA.
Investigative Ophthalmology & Visual Science May 1996, Vol.37, 1084-1096. doi:
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    • Get Citation

      D B Gervin, G M Cann, D O Clegg; Temporal and spatial regulation of integrin vitronectin receptor mRNAs in the embryonic chick retina.. Invest. Ophthalmol. Vis. Sci. 1996;37(6):1084-1096.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To identify integrin vitronectin receptor subunit mRNAs in the developing avian retina and to track their expression. METHODS: Reverse transcription-polymerase chain reaction was used to identify integrin vitronectin receptor subunit mRNAs expressed in embryonic chick retina. cDNA clones encoding the beta 5 subunit were isolated and sequenced. Expression patterns of mRNAs encoding alpha v, beta 3, and beta 5 subunits were analyzed using northern analysis and in situ hybridization. RESULTS: Integrin beta 1, beta 3, and beta 5 subunit mRNA were identified in embryonic day 6 chick retina. The sequence of chicken beta 5 was 77% identical to that of human beta 5, and sequences with known signaling functions were highly conserved. Integrin alpha v, beta 3, and beta 5 mRNAs were expressed throughout the development of the embryonic retina, with the highest levels per retina observed around the embryonic day 9. In situ hybridization showed that both beta 3 and beta 5 were expressed throughout the developing retina, particularly in undifferentiated neuroepithelial precursor cells. At later times, beta 3 was expressed uniformly throughout the retina, whereas beta 5 expression was highest in a band throughout the central retina. CONCLUSIONS: The strong conservation of sequences with known signaling functions in chicken beta 5 suggests that it functions in a manner similar to human beta 5. Spatial expression patterns of vitronectin receptor subunits at early times of development point to a range of possible functions beyond axon outgrowth, including retinoblast proliferation, adhesion, and migration.

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