June 1996
Volume 37, Issue 7
Free
Articles  |   June 1996
Morphology of the normal human lens.
Author Affiliations
  • V L Taylor
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599-7090, USA.
  • K J al-Ghoul
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599-7090, USA.
  • C W Lane
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599-7090, USA.
  • V A Davis
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599-7090, USA.
  • J R Kuszak
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599-7090, USA.
  • M J Costello
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599-7090, USA.
Investigative Ophthalmology & Visual Science June 1996, Vol.37, 1396-1410. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      V L Taylor, K J al-Ghoul, C W Lane, V A Davis, J R Kuszak, M J Costello; Morphology of the normal human lens.. Invest. Ophthalmol. Vis. Sci. 1996;37(7):1396-1410.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: To provide a quantitative, morphologic description of differentiated lens fiber cells in all regions of aged normal human lenses. METHODS: Transparent normal human lenses (age range, 44 to 71 years) were examined with correlative transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Vibratome sections allowed examination of internal structures, whereas dissected whole lenses revealed surface characteristics. Additionally, image analysis was used to measure cross-sectional areas of fiber cells. RESULTS: Approximate regional dimensions (percentage of diameter and thickness, respectively) were determined for whole lenses: cortex 16%, 17%; adult nucleus 24%, 21%; juvenile nucleus 12%, 9%; fetal nucleus 45%, 49%; and embryonic nucleus 3%, 4%. Cortical cells were irregularly hexagonal, and the average cross-sectional area measured 24 +/- 9 microns2. Adult nuclear cells were flattened with intricate membranous interdigitations and an area of 7 +/- 2 microns2. Juvenile nuclear cells had an area of 14 +/- 5 microns2. Fetal nuclear cells were rounded with an area of 35 +/- 22 microns2. Embryonic nuclear cells also were rounded and had a variable area of 80 +/- 68 microns2. Fiber cell cytoplasm in all lens regions appeared smooth in texture and homogeneous in staining density. CONCLUSIONS: Both TEM and SEM are necessary to obtain a complete description of fiber cells. Cross-sections of fibers give new insights into the lamellar organization of the lens, indicating that each region has characteristic cell shapes and sizes. Furthermore, average dimensions were used to demonstrate that the number of cells and approximate growth rates vary significantly between adjacent regions.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×