May 1996
Volume 37, Issue 6
Free
Articles  |   May 1996
Intraocular pressure measurement in cynomolgus monkeys. Tono-Pen versus manometry.
Author Affiliations
  • J A Peterson
    Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, Madison 53792-3220, USA.
  • J A Kiland
    Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, Madison 53792-3220, USA.
  • M A Croft
    Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, Madison 53792-3220, USA.
  • P L Kaufman
    Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, Madison 53792-3220, USA.
Investigative Ophthalmology & Visual Science May 1996, Vol.37, 1197-1199. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J A Peterson, J A Kiland, M A Croft, P L Kaufman; Intraocular pressure measurement in cynomolgus monkeys. Tono-Pen versus manometry.. Invest. Ophthalmol. Vis. Sci. 1996;37(6):1197-1199.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: In living cynomolgus monkey eyes, the authors compared manometrically set and measured intraocular pressure (IOP) with simultaneous IOP readings obtained with the Tono-Pen (TP), a handheld applanation tonometer based on the Mackay-Marg principle. METHODS: In three pentobarbital-anesthetized cynomolgus monkeys, IOP was set and measured manometrically after anterior chamber cannulation through the peripheral cornea with a 26-gauge needle connected to a vertically adjustable reservoir and a pressure transducer. Intraocular pressure was raised in approximately 5 mm Hg steps from 5 mm Hg to 60 mm Hg and then lowered in 5 mm Hg steps to 5 mm Hg, with TP measurements taken at each increment and decrement in open and stopcock modes. RESULTS: Linear regression analysis of TP on manometric readings for grouped data from all six eyes, with each data point representing the average of all the TP readings from one eye at each manometric pressure setting, showed a slope 0.692 +/- 0.016 and 0.683 +/- 0.023 (both significantly different from 1; P < 0.001), intercept 1.21 +/- 0.60 and 1.64 +/- 0.82 mm Hg (both significantly different from 0.0, P < 0.05), and correlation coefficient 0.981 and 0.96 in open stopcock and closed stopcock mode, respectively. There were no striking differences when the data were analyzed for individual eyes or animals, for open versus closed stopcock manometry, or for increasing versus decreasing manometric IOP. CONCLUSIONS: The TP provides reproducible measurements of IOP in cynomolgus monkeys, with measurement accuracy dependent on the generation of an appropriate calibration curve.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×