June 1996
Volume 37, Issue 7
Free
Articles  |   June 1996
Retinal Thy-1 expression during development.
Author Affiliations
  • C J Liu
    Department of Ophthalmology, Massachuselts Eye & Ear Infirmary, Harvard Medical School, Boston 02114, USA.
  • N Chaturvedi
    Department of Ophthalmology, Massachuselts Eye & Ear Infirmary, Harvard Medical School, Boston 02114, USA.
  • C J Barnstable
    Department of Ophthalmology, Massachuselts Eye & Ear Infirmary, Harvard Medical School, Boston 02114, USA.
  • E B Dreyer
    Department of Ophthalmology, Massachuselts Eye & Ear Infirmary, Harvard Medical School, Boston 02114, USA.
Investigative Ophthalmology & Visual Science June 1996, Vol.37, 1469-1473. doi:
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      C J Liu, N Chaturvedi, C J Barnstable, E B Dreyer; Retinal Thy-1 expression during development.. Invest. Ophthalmol. Vis. Sci. 1996;37(7):1469-1473.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To evaluate the developmental expression of Thy-1 in the retina. Thy-1, the most abundant mammalian neuronal surface glycoprotein, is likely to play a significant role in retinal development. In the mammalian retina, it is found predominantly, if not exclusively, on retinal ganglion cells. METHODS: Rat retinae of various ages were stained immunohistochemically for Thy-1 with 2G12, a monoclonal Thy-1 antibody. Sections were analyzed digitally to quantify bound antibody. Using semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), the expression of Thy-1 protein was compared with the levels of mRNA detected. RESULTS: Thy-1-dependent fluorescence was detected in rat retinae from birth, albeit at low levels. Thy-1 labeling was localized predominantly to the ganglion cell layer. Minimal, fine patterns of linear and reticular fluorescence were noted in the inner nuclear layer. Thy-1 levels reached a maximal level at approximately postnatal day 14. RT-PCR measurements showed a similar time course for the increase in Thy-1 expression. CONCLUSIONS: The Thy-1 antigen is present in the inner retina at birth. Its level increases steadily after birth and peaks during the second week of life. Thy-1 expression is approximately coterminous with synaptogenesis of the inner plexiform layer and may play a role in synaptogenesis of the inner retina or in other developmental milestones in the formation of the visual system.

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